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A tetracycline-repr...
A tetracycline-repressible transactivator system to study essential genes in malaria parasites
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Pino, Paco (author)
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Sebastian, Sarah (author)
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Kim, Eunbin Arin (author)
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Bush, Erin (author)
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Brochet, Mathieu (author)
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Volkmann, Katrin (author)
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Kozlowski, Elyse (author)
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Llinás, Manuel (author)
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- Billker, Oliver (author)
- Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
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Soldati-Favre, Dominique (author)
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(creator_code:org_t)
- Elsevier, 2012
- 2012
- English.
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In: Cell Host and Microbe. - : Elsevier. - 1931-3128 .- 1934-6069. ; 12:6, s. 824-834
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Abstract
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- A major obstacle in analyzing gene function in apicomplexan parasites is the absence of a practical regulatable expression system. Here, we identified functional transcriptional activation domains within Apicomplexan AP2 (ApiAP2) family transcription factors. These ApiAP2 transactivation domains were validated in blood-, liver-, and mosquito-stage parasites and used to create a robust conditional expression system for stage-specific, tetracycline-dependent gene regulation in Toxoplasma gondii, Plasmodium berghei, and Plasmodium falciparum. To demonstrate the utility of this system, we created conditional knockdowns of two essential P. berghei genes: profilin (PRF), a protein implicated in parasite invasion, and N-myristoyltransferase (NMT), which catalyzes protein acylation. Tetracycline-induced repression of PRF and NMT expression resulted in a dramatic reduction in parasite viability. This efficient regulatable system will allow for the functional characterization of essential proteins that are found in these important parasites.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Pino, Paco
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Sebastian, Sarah
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Kim, Eunbin Arin
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Bush, Erin
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Brochet, Mathieu
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Volkmann, Katrin
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show more...
-
Kozlowski, Elyse
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Llinás, Manuel
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Billker, Oliver
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Soldati-Favre, D ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Cell Host and Mi ...
- By the university
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Umeå University