Sökning: WFRF:(Svensson Lennart 1954 )
> Borén Thomas >
Lactobacilli expres...
Lactobacilli expressing variable domain of llama heavy-chain antibody fragments (lactobodies) confer protection against rotavirus-induced diarrhea
-
Pant, Neha (författare)
-
Hultberg, Anna (författare)
-
Zhao, Yaofeng (författare)
-
visa fler...
-
- Svensson, Lennart, 1954- (författare)
- Linköpings universitet,Hälsouniversitetet,Molekylär virologi
-
Pan-Hammarström, Qiang (författare)
-
Johansen, Kari (författare)
-
Pouwels, Peter H (författare)
-
Ruggeri, Franco M (författare)
-
Hermans, Pim (författare)
-
Frenken, Leon (författare)
-
Borén, Thomas (författare)
-
Marcotte, Harold (författare)
-
Hammarström, Lennart (författare)
-
visa färre...
-
(creator_code:org_t)
- 2006
- 2006
- Engelska.
-
Ingår i: Journal of Infectious Diseases. - 0022-1899 .- 1537-6613. ; 194:11, s. 1580-1588
- Relaterad länk:
-
https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Background. Rotavirus-induced diarrhea poses a worldwide medical problem in causing substantial morbidity and mortality among children in developing countries. We therefore developed a system for passive immunotherapy in which recombinant lactobacilli constitutively express neutralizing variable domain of llama heavy-chain (VHH) antibody fragments against rotavirus. Methods. VHH were expressed in Lactobacillus paracasei, in both secreted and cell surface-anchored forms. Electron microscopy was used to investigate the binding efficacy of VHH-expressing lactobacilli. To investigate the in vivo function of VHH-expressing lactobacilli, a mouse pup model of rotavirus infection was used. Results. Efficient binding of the VHH antibody fragments to rotavirus was shown by enzyme-linked immunosorbent assay and scanning electron microscopy. VHH fragments expressed by lactobacilli conferred a significant reduction in infection in cell cultures. When administered orally, lactobacilli-producing surface-expressed VHH markedly shortened disease duration, severity, and viral load in a mouse model of rotavirus-induced diarrhea when administered both fresh and in a freeze-dried form. Conclusions. Transformed lactobacilli may form the basis of a novel form of prophylactic treatment against rotavirus infections and other diarrheal diseases. © 2006 by the Infectious Diseases Society of America. All rights reserved.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Pant, Neha
-
Hultberg, Anna
-
Zhao, Yaofeng
-
Svensson, Lennar ...
-
Pan-Hammarström, ...
-
Johansen, Kari
-
visa fler...
-
Pouwels, Peter H
-
Ruggeri, Franco ...
-
Hermans, Pim
-
Frenken, Leon
-
Borén, Thomas
-
Marcotte, Harold
-
Hammarström, Len ...
-
visa färre...
- Artiklar i publikationen
-
Journal of Infec ...
- Av lärosätet
-
Linköpings universitet