| 1. |
- Lard, Mercy, et al.
(författare)
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Use of dielectrophoresis for directing T cells to microwells before nanostraw transfection : modelling and experiments
- 2022
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Ingår i: RSC Advances. - : Royal Society of Chemistry (RSC). - 2046-2069. ; 12:47, s. 30295-30303
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Tidskriftsartikel (refereegranskat)abstract
- Nanostraw substrates have great potential for achieving minimally invasive cell transfection. Cells located on the nanostraw substrate are subjected to mild DC electric pulses applied across the nanostraw substrate, which open pores in the cell membrane on top of the nanostraws and drives charged cargo through these pores via electrophoresis. However, with this method, the current may leak through uncovered nanostraws, thereby decreasing the desired effect in the cell-covered nanostraws. A minimization of the number of uncovered nanostraws could be achieved by high cell coverage, but this is challenging when working with small cell populations. Nanostraw substrates of smaller area could be covered by smaller cell populations but are hard to integrate into fluidics systems. Here, we use simulations and experiments to show that this issue can be addressed by covering the nanostraw substrate with an insulating layer containing pores of similar size to cells. The pores act as traps into which cells can be guided using dielectrophoresis, ensuring a high degree of occupancy while maintaining a high cell viability, even if the total number of cells is low.
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| 2. |
- Persson, Henrik, et al.
(författare)
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From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.
- 2015
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 5
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Tidskriftsartikel (refereegranskat)abstract
- The field of vertical nanowire array-based applications in cell biology is growing rapidly and an increasing number of applications are being explored. These applications almost invariably rely on the physical properties of the nanowire arrays, creating a need for a better understanding of how their physical properties affect cell behaviour. Here, we investigate the effects of nanowire density on cell migration, division and morphology for murine fibroblasts. Our results show that few nanowires are sufficient to immobilize cells, while a high nanowire spatial density enables a "bed-of-nails" regime, where cells reside on top of the nanowires and are fully motile. The presence of nanowires decreases the cell proliferation rate, even in the "bed-of-nails" regime. We show that the cell morphology strongly depends on the nanowire density. Cells cultured on low (0.1 μm(-2)) and medium (1 μm(-2)) density substrates exhibit an increased number of multi-nucleated cells and micronuclei. These were not observed in cells cultured on high nanowire density substrates (4 μm(-2)). The results offer important guidelines to minimize cell-function perturbations on nanowire arrays. Moreover, these findings offer the possibility to tune cell proliferation and migration independently by adjusting the nanowire density, which may have applications in drug testing.
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| 3. |
- Persson, Henrik, et al.
(författare)
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Vertical nanotubes connected by a subsurface nanochannel
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; 3, s. 1862-1864
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Konferensbidrag (refereegranskat)abstract
- We have built an array of vertical nanotubes connected by a subsurface nanofluidic channel. The nanotube system is to be used for cell injections. By culturing cells on top of the nanotubes the cells will be pierced and molecules can enter the cells via the subsurface channel. Several channels can be made on the same sample and different molecules can be injected into different subpopulations of cells. The advantages of our system over similar injection devices include spatial and temporal resolution as well as an increased choice of injected molecules.
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| 4. |
- Sköld, Niklas, et al.
(författare)
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Nanofluidics in hollow nanowires
- 2010
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Ingår i: NANOTECHNOLOGY. - : IOP Publishing. - 0957-4484 .- 1361-6528. ; 21:15
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Tidskriftsartikel (refereegranskat)abstract
- We present a novel scheme for producing nanotube membranes using free-standing hollow nanowires, with easily controllable dimensions. GaAs–AlInP core–shell nanowires were grown by metal–organic vapor phase epitaxy and were partially embedded in a polymer film. The GaAs core and substrate were etched selectively, leaving tubes with open access to both sides of the membrane. Electrophoretic transport of T4-phage DNA through the hollow nanowires was demonstrated using epifluorescence microscopy.
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