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Identification of a...
Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation : evidence for an association involving the MUC5B mucin
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Thornton, David (author)
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- Davies, Julia (author)
- Malmö högskola,Odontologiska fakulteten (OD)
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Kirkham, Sara (author)
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Gautrey, Alex (author)
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Khan, Nagma (author)
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Richardson, Paul (author)
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Sheehan, John (author)
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(creator_code:org_t)
- Oxford Journals, 2001
- 2001
- English.
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In: Glycobiology. - : Oxford Journals. - 0959-6658 .- 1460-2423. ; 11:11, s. 969-977
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Rate-zonal centrifugation of a reduced and alkylated respiratory mucin preparation identified a protein-rich fraction. This was subjected to trypsin treatment and one of the many liberated peptides was purified and its N-terminal sequence determined. The peptide was identical to a 14 amino acid sequence from the scavenger receptor cysteine-rich domain containing glycoprotein gp-340. A polyclonal antiserum, raised against the peptide, stained the serous cells in the submucosal glands of human tracheal tissue. The glycoprotein was purified from respiratory mucus by density-gradient centrifugation, gel chromatography, and anion exchange chromatography. The molecule exhibited a heterogeneous distribution of buoyant density (1.28-1.46 g/ml) that overlapped with the gel-forming mucins, was included on Sepharose CL-2B and was quite highly anionic. SDS-PAGE indicated a mass greater than 208 kDa and measurements performed across the molecular size distribution indicated an average M(r) of 5 x 10(5) with a range of M(r) from 2 x 10(5) to 1 x 10(6). Gel chromatography of respiratory mucus extracts ("associative" and "dissociative") indicated that this glycoprotein forms complexes that may involve the large gel-forming mucins MUC5AC and MUC5B. Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Gp340
- Mucus
- MUC5B
- MUC5AC
Publication and Content Type
- ref (subject category)
- art (subject category)
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