| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Fenstermacher, M.E., et al.
(författare)
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DIII-D research advancing the physics basis for optimizing the tokamak approach to fusion energy
- 2022
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Ingår i: Nuclear Fusion. - : IOP Publishing. - 0029-5515 .- 1741-4326. ; 62:4
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Tidskriftsartikel (refereegranskat)abstract
- DIII-D physics research addresses critical challenges for the operation of ITER and the next generation of fusion energy devices. This is done through a focus on innovations to provide solutions for high performance long pulse operation, coupled with fundamental plasma physics understanding and model validation, to drive scenario development by integrating high performance core and boundary plasmas. Substantial increases in off-axis current drive efficiency from an innovative top launch system for EC power, and in pressure broadening for Alfven eigenmode control from a co-/counter-I p steerable off-axis neutral beam, all improve the prospects for optimization of future long pulse/steady state high performance tokamak operation. Fundamental studies into the modes that drive the evolution of the pedestal pressure profile and electron vs ion heat flux validate predictive models of pedestal recovery after ELMs. Understanding the physics mechanisms of ELM control and density pumpout by 3D magnetic perturbation fields leads to confident predictions for ITER and future devices. Validated modeling of high-Z shattered pellet injection for disruption mitigation, runaway electron dissipation, and techniques for disruption prediction and avoidance including machine learning, give confidence in handling disruptivity for future devices. For the non-nuclear phase of ITER, two actuators are identified to lower the L-H threshold power in hydrogen plasmas. With this physics understanding and suite of capabilities, a high poloidal beta optimized-core scenario with an internal transport barrier that projects nearly to Q = 10 in ITER at ∼8 MA was coupled to a detached divertor, and a near super H-mode optimized-pedestal scenario with co-I p beam injection was coupled to a radiative divertor. The hybrid core scenario was achieved directly, without the need for anomalous current diffusion, using off-axis current drive actuators. Also, a controller to assess proximity to stability limits and regulate β N in the ITER baseline scenario, based on plasma response to probing 3D fields, was demonstrated. Finally, innovative tokamak operation using a negative triangularity shape showed many attractive features for future pilot plant operation.
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| 4. |
- Sun, Fengbo, et al.
(författare)
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1,5-Diiodocycloctane: a cyclane solvent additive that can extend the exciton diffusion length in thick film organic solar cells
- 2024
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Ingår i: Energy and Environmental Sciences. - 1754-5692 .- 1754-5706. ; 17:5, s. 1916-1930
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Tidskriftsartikel (refereegranskat)abstract
- The short exciton diffusion length associated with most state-of-the-art organic semiconductors used in organic solar cells (OSCs) imposes severe limits on the exciton transport in the larger donor/acceptor domains and the exciton dissociation at the interface, which hinder further improvements in the power conversion efficiencies (PCE) of the thick-film devices. In this study, a new cyclane, 1,5-diiodocycloctane (DICO), was employed as a solvent additive to effectively extend the exciton LD within the bulk-heterojunction blend, which can function with the multiple photovoltaic materials system. Due to the great enhancement of molecular stacking and exclusively large domain sizes of photovoltaic materials with the assistance of the DICO additive, the trap density in devices is significantly reduced, thereby nearly doubling the LD in the thick film OSCs. Notably, the DICO-processed PM6/L8-BO-based OSC showed high thickness tolerance for the bulk-heterojunction (BHJ) layer, delivering a high PCE of 19.1% in the case of a 110 nm thick film and still maintaining an excellent PCE of 17.2% in the case of a 300 nm thick film. Crucially, a noticeably increased stability of the multiple materials system was observed in the DICO-processed OSCs. These findings enrich the additive family with new cyclane systems to extend the exciton LD in thick film OSCs with high performance.
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| 5. |
- Deng, Liying, et al.
(författare)
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KCl acts as a flux to assist the growth of sub-millimeter-scale metallic 2D non-layered molybdenum dioxide
- 2024
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Ingår i: Rare Metals. - 1001-0521 .- 1867-7185. ; In Press
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Tidskriftsartikel (refereegranskat)abstract
- Two-dimensional (2D) metal oxides (2DMOs), such as MoO2, have made impressive strides in recent years, and their applicability in a number of fields such as electronic devices, optoelectronic devices and lasers has been demonstrated. However, 2DMOs present challenges in their synthesis using conventional methods due to their non-van der Waals nature. We report that KCl acts as a flux to prepare large-area 2DMOs with sub-millimeter scale. We systematically investigate the effects of temperature, homogeneous time and cooling rate on the products in the flux method, demonstrating that in this reaction a saturated homogenous solution is obtained upon the melting of the salt and precursor. Afterward, the cooling rate was adjusted to regulate the thickness of the target crystals, leading to the precipitation of 2D non-layered material from the supersaturated solution; by applying this method, the highly crystalline non-layered 2D MoO2 flakes with so far the largest lateral size of up to sub-millimeter scale (~ 464 μm) were yielded. Electrical studies have revealed that the 2D MoO2 features metallic properties, with an excellent sheet resistance as low as 99 Ω·square−1 at room temperature, and exhibits a property of charge density wave in the measurement of resistivity as a function of temperature. Graphical abstract: TOC (Table of Content) (Figure presented.)
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| 6. |
- Li, Xun, et al.
(författare)
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Investigation of burning velocity of lean and rich premixed NH3/H2 turbulent flames using multi-scalar imaging
- 2024
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Ingår i: Proceedings of the Combustion Institute. - 1540-7489. ; 40:1-4
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Tidskriftsartikel (refereegranskat)abstract
- Two groups of both lean and rich NH3/H2/O2/N2 turbulent premixed piloted jet flames with the same laminar flame speed are investigated using simultaneous multi-scalar laser diagnostics techniques including NH3/NH/OH planar laser induced fluorescence (PLIF) and Rayleigh scattering. One group uses air as the oxidizer and the other uses an adapted mixture of atomic N-to-H ratio of 1:3 associated with in-situ hydrogen production from ammonia cracking. Turbulent rms velocity uʹ is varied in a wide range by changing the bulk flow velocity. Global consumption speeds ST,G are evaluated by measuring the inlet bulk flow rates of reactants and areas of time-averaged flame fronts extracted using different flame marker contours, i.e., cNH3 = 0.3, cNH = 0.3 (inner contour), and cOH = 0.4, where c designates species number density normalized using its maximum value. The obtained results show the following trends to be emphasized. First, an increase in ST,G,NH3 by uʹ is more pronounced when compared to two other global consumption speeds (ST,G,NHi , where i indicates the inner contour, and ST,G,OH ) and is close to a linear dependence. The adapted mixture shows only a moderate influence on ST,G,NH3 but not on the other two. Second, ST,G,NH3 is weakly affected by variations in Lewis number Le. On the contrary, third, ST,G,NHi is significantly higher in lean mixtures characterized by Le < 1 when compared to rich mixtures characterized by Le > 1. Fourth, these variations in ST,G,NHi are reasonably well approximated introducing an empirical factor of Le−0.9 into fitting ST,G,NHi /SL onto uʹ/SL . The qualitative difference between sensitivities of ST,G,NH3 and ST,G,NHi to variations in Le is attributed to the fact that the NH3 and NH isosurfaces are localized to preheat and reaction zones, respectively, of the laminar flames. The reported experimental data imply different influence of differential diffusion on flame preheat and reaction zones, thus, calling for further research into the issue.
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| 7. |
- Liang, Xifeng, et al.
(författare)
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On the aerodynamic loads when a high speed train passes under an overhead bridge
- 2020
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Ingår i: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 202
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Tidskriftsartikel (refereegranskat)abstract
- The aerodynamic loads on the overhead bridge bottom surface induced by train passage are reported in this paper. Both moving model test and numerical simulation approaches at the 1:20 scale are used. The numerical work is validated through both mesh independence tests and comparison with experimental data. Typical pressure variation curves are plotted and compared with previous studies. The peak pressure values’ dependence on the Reynolds number is considered through four sets of experiments with different train running speeds. The peak pressure coefficient distribution law for the bridge bottom surface is presented. Differences in the pressure distribution in different bridge bottom areas are explained based on more detailed flow field information. The influence of the bridge height on the aerodynamic load magnitude and time interval is presented. Moreover, the application of the CEN Standard to practical engineering issues is discussed.
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| 8. |
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| 9. |
- Chen, Guang, et al.
(författare)
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Dynamic analysis of the effect of nose length on train aerodynamic performance
- 2019
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Ingår i: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 184, s. 198-208
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Tidskriftsartikel (refereegranskat)abstract
- The improved delayed detached eddy simulation (IDDES) was used to study the influence of the train’s nose length on its aerodynamic performance. Both the time-averaged and instantaneous near-wake structures and the associated distribution of slipstream velocity are compared for three nose lengths. As the nose length increases, the mean and Std values of the drag and lift force are decreased. The shorter nose-length case results in a higher slipstream velocity. In particular, at the TSI track-side position, the TSI value U_2δ for the 5-m nose length case is 30% and 32% higher than the corresponding values for the 7.5-m and 10-m nose length cases, respectively. The dynamical flow topology in the wake reveals that the flow structures of the 5-m nose length are different from those of the other two cases in the tail streamline surface. As nose length increases, the longitudinal vortices are weaker, and the angle and distance between the longitudinal vortices are smaller. The shear production from the P_xy caused by the separation of the boundary layer at the lateral wall of the tail train is greater than that of the P_xz caused by the separation of the boundary layer at the top and bottom of the tail train.
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| 10. |
- Fan, Qunping, 1989, et al.
(författare)
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High-performance all-polymer solar cells enabled by a novel low bandgap non-fully conjugated polymer acceptor
- 2021
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Ingår i: Science in China Series B. - : Springer Nature. - 1674-7291 .- 1869-1870. ; 64, s. 1380-1388
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Tidskriftsartikel (refereegranskat)abstract
- Anon-fully conjugated polymer as a new class of acceptor materials has shown some advantages over its small molecular counterpart when used in photoactive layers for all-polymer solar cells (all-PSCs), despite a low power conversion efficiency (PCE) caused by its narrow absorption spectra. Herein, a novel non-fully conjugated polymer acceptor PFY-2TS with a low bandgap of similar to 1.40 eV was developed, via polymerizing a large pi-fused small molecule acceptor (SMA) building block (namely YBO) with a non-conjugated thioalkyl linkage. Compared with its precursor YBO, PFY-2TS retains a similar low bandgap but a higher LUMO level. Moreover, compared with the structural analog of YBO-based fully conjugated polymer acceptor PFY-DTC, PFY-2TS shows similar absorption spectrum and electron mobility, but significantly different molecular crystallinity and aggregation properties, which results in optimal blend morphology with a polymer donor PBDB-T and better device physical processes in all-PSCs. As a result, PFY-2TS-based all-PSCs achieved a PCE of 12.31% with a small energy loss of 0.56 eV enabled by the reduced non-radiative energy loss (0.24 eV), which is better than that of 11.08% for the PFY-DTC-based ones. Our work clearly demonstrated that non-fully conjugated polymers as a new class of acceptor materials are very promising for the development of high-performance all-PSCs.
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