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Sökning: WFRF:(Wieland T) > RISE

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1.
  • Paulraj, Thomas, et al. (författare)
  • Primary cell wall inspired micro containers as a step towards a synthetic plant cell
  • 2020
  • Ingår i: Nature Communications. - : Nature Research. - 2041-1723. ; 11:1
  • Tidskriftsartikel (refereegranskat)abstract
    • The structural integrity of living plant cells heavily relies on the plant cell wall containing a nanofibrous cellulose skeleton. Hence, if synthetic plant cells consist of such a cell wall, they would allow for manipulation into more complex synthetic plant structures. Herein, we have overcome the fundamental difficulties associated with assembling lipid vesicles with cellulosic nanofibers (CNFs). We prepare plantosomes with an outer shell of CNF and pectin, and beneath this, a thin layer of lipids (oleic acid and phospholipids) that surrounds a water core. By exploiting the phase behavior of the lipids, regulated by pH and Mg2+ ions, we form vesicle-crowded interiors that change the outer dimension of the plantosomes, mimicking the expansion in real plant cells during, e.g., growth. The internal pressure enables growth of lipid tubules through the plantosome cell wall, which paves the way to the development of hierarchical plant structures and advanced synthetic plant cell mimics. © 2020, The Author(s).
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2.
  • Wieland, D. C. F., et al. (författare)
  • Complex solutions under shear and pressure : A rheometer setup for X-ray scattering experiments
  • 2017
  • Ingår i: Journal of Synchrotron Radiation. - : International Union of Crystallography. - 0909-0495 .- 1600-5775. ; 24, s. 646-652
  • Tidskriftsartikel (refereegranskat)abstract
    • A newly developed high-pressure rheometer for in situ X-ray scattering experiments is described. A commercial rheometer was modified in such a way that X-ray scattering experiments can be performed under different pressures and shear. First experiments were carried out on hyaluronan, a ubiquitous biopolymer that is important for different functions in the body such as articular joint lubrication. The data hint at a decreased electrostatic interaction at higher pressure, presumably due to the increase of the dielectric constant of water by 3% and the decrease of the free volume at 300 bar.
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