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Sökning: WFRF:(Wiklund T) > Kungliga Tekniska Högskolan

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  • Nilsson, H., et al. (författare)
  • Microparticles for selective protein determination in capillary electrophoresis
  • 2001
  • Ingår i: Electrophoresis. - 0173-0835 .- 1522-2683. ; 22:12, s. 2384-2390
  • Tidskriftsartikel (refereegranskat)abstract
    • A system for detection of trace amounts of protein was developed. Two different monoclonal antibodies against human chorionic gonadotropin (hCG) were covalently bound to latex particles. When the latex particles were mixed with a sample containing hCG, a latex-protein-latex complex (immunocomplex) was formed. The complex was separated from the single latex particles using capillary electrophoresis and detected using UV-Vis detection. Limit of detection was 8 amol hCG. The separation was also monitored in real time using laser induced fluorescence - charge coupled device (LIF-CCD) imaging detection. However, a limitation of the method is the restriction to detection of proteins for which monoclonal antibodies are available.
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3.
  • Nilsson, Måns, et al. (författare)
  • Analytical framework and tool kit for SEA follow-up
  • 2009
  • Ingår i: Environmental impact assessment review. - : Elsevier BV. - 0195-9255 .- 1873-6432. ; 29:3, s. 186-199
  • Tidskriftsartikel (refereegranskat)abstract
    • Most Strategic Environmental Assessment (SEA) research and applications have so far neglected the ex post stages of the process, also called SEA follow-up. Tool kits and methodological frameworks for engaging effectively with SEA follow-up have been conspicuously missing. In particular. little has so far been learned from the much more mature evaluation literature although many aspects are similar. This paper provides an analytical framework and tool kit for SEA follow-up. It is based on insights and tools developed within programme evaluation and environmental systems analysis. It is also grounded in empirical studies into real planning and programming practices at the regional level, but should have relevance for SEA processes at all levels. The purpose of the framework is to promote a learning-oriented and integrated use of SEA follow-up in strategic decision making. It helps to identify appropriate tools and their use in the process, and to systematise the use of available data and knowledge across the planning organization and process. It distinguishes three stages in follow-up: scoping, analysis and learning, identifies the key functions and demonstrates the informational linkages to the strategic decision-making process. The associated tool kit includes specific analytical and deliberative tools. Many of these are applicable also ex ante. but are then used in a predictive mode rather than on the basis of real data. The analytical element of the framework is organized on the basis of programme theory and "DPSIR" tools. The paper discusses three issues in the application of the framework: understanding the integration of organizations and knowledge; understanding planners' questions and analytical requirements; and understanding interests, incentives and reluctance to evaluate.
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4.
  • Olofsson, K., et al. (författare)
  • Unanchored micro-tumors in an ultrasonic actuated multi-well microplate with protein repellent coating
  • 2016
  • Ingår i: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. - : Chemical and Biological Microsystems Society. - 9780979806490 ; , s. 409-410
  • Konferensbidrag (refereegranskat)abstract
    • In this paper we demonstrate an improved tissue engineering method producing 100 three-dimensional (3D) HepG2 cell structures in parallel based on a combination of ultrasonic actuation and polymer coating in a multi-well microplate. By the use of a polymer coating in the plates, the method creates non-adherent tumor models of controlled size and shape which introduces both a more flexible 3D culture system as well as improved quality of the 3D tumor relative to previous studies [1].
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  • Resultat 1-5 av 5

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