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Sökning: WFRF:(Yang Yong jian) > Chalmers tekniska högskola

  • Resultat 1-4 av 4
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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • 2019
  • Tidskriftsartikel (refereegranskat)
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3.
  • Fan, Jin, et al. (författare)
  • Investigation of ultra-wideband Bowtie antennas for phased array feed application
  • 2020
  • Ingår i: International Journal of RF and Microwave Computer-Aided Engineering. - : Hindawi Limited. - 1096-4290 .- 1099-047X. ; 30:11
  • Tidskriftsartikel (refereegranskat)abstract
    • In this article, two new ultra-wideband (UWB) dual-polarized Bowtie antennas are investigated as the elements for a phased array feed for reflectors. In addition to its UWB impedance matching characteristic, the Bowtie antennas have stable large beam-width and a low cross-polar level over a wide frequency band with a compact size, which is an essence for phased array applications. The simulated and measured results state a low ohmic loss, good impedance matching (S11 below −15 dB) and good radiation performance, with a simple structure for easy manufacturing. The proposed antennas can be good candidates for phased array feed (PAF) in FAST and the SKA (square kilometer array) pathfinder PHAROS2 projects, and massive MIMO antennas in wireless communication systems.
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4.
  • Kang, Jian, et al. (författare)
  • Vegetation in urban streets, squares, and courtyards
  • 2014
  • Ingår i: Environmental Methods for Transport Noise Reduction. - : CRC Press. - 9781482288773 ; , s. 177-194
  • Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
    • One of various ways in which vegetation cover used in the greening of urban areas can help improve the health and well-being of people is in how it changes the acoustic environment. This chapter presents findings of computer simulations and scale modelling to examine and quantify the effectiveness of green roof and green wall (vertical garden) systems in reducing road traffic noise for streets, squares, and roadside courtyards. Noise reduction by sound absorption in reflected and diffracted (over roofs) sound paths is investigated. Particular attention is paid to the importance of vegetation placement relative to the receiver/listening positions. Because the soil substrate used for the vertical walls has good sound absorption properties, it also can be used for green barriers. In this chapter, the effects of a low barrier made of green wall substrate are studied for an installation on the ground and on the top of buildings surrounding a courtyard.
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