| 1. |
- Ye, Z., et al.
(författare)
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Design for 2D anisotropic photonic crystal with large absolute band gaps by using a genetic algorithm
- 2004
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Ingår i: European Physical Journal B. - : Springer Science and Business Media LLC. - 1434-6028 .- 1434-6036. ; 37:4, s. 417-419
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Tidskriftsartikel (refereegranskat)abstract
- A 2-state genetic algorithm (GA) approach is used to design a two-dimensional (2D) anisotropic photonic crystal of square lattice with a maximal absolute band gap. The unit cell is devided equally into many small squares, and each filling pattern of squares with two dielectric materials corresponds to a binary number. As a numerical example, the GA gives a 2D Te structure with a relative width of the absolute band gap of about 23%. After a further optimization, a new structure is obtained with a relative width of the absolute band gap of about 28%.
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| 2. |
- Surugiu, Ioana, et al.
(författare)
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Chemiluminescence imaging ELISA using an imprinted polymer as the recognition element instead of an antibody
- 2001
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 73:3, s. 487-491
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Tidskriftsartikel (refereegranskat)abstract
- An imaging assay analogous to competitive enzyme immunoassays has been developed using a molecularly imprinted polymer instead of an antibody. The antigen 2,4-dichlorophenoxyacetic acid (2,4-D) was labeled with tobacco peroxidase, and the chemiluminescence reaction of luminol was used for detection. Microtiter plates (96 or 384 wells) were coated with polymer microspheres imprinted with 2,4-D, which were fixed in place by using poly(vinyl alcohol) as glue. In a competitive mode, the analyte-peroxidase conjugate was incubated with the free analyte in the microtiter plate, after which the bound fraction of the conjugate was quantified. After addition of the chemiluminescent substrates, light emission was measured in a high-throughput imaging format with a CCD camera. Calibration curves corresponding to analyte concentrations ranging from 0.01 to 100 μg/mL were obtained.
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| 3. |
- Surugiu, Ioana, et al.
(författare)
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Development of a flow injection capillary chemiluminescent ELISA using an imprinted polymer instead of the antibody
- 2001
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 73:17, s. 4388-4392
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Tidskriftsartikel (refereegranskat)abstract
- A flow injection competitive assay analogous to enzyme immunoassays has been developed using a molecularly imprinted polymer instead of the antibody. A glass capillary was modified by covalently attaching an imprinted polymer to the inner capillary wall. The herbicide 2,4-dichlorophenoxyacetic acid was used as a model analyte. The analyte was labeled with tobacco peroxidase, and chemiluminescence was used for detection in combination with a photomultiplier tube or a CCD camera. In a competitive mode, the analyte-peroxidase conjugate was passed together with the free analyte through the polymer-coated capillary mounted in a flow system. After a washing step, the chemiluminescent substrate was injected and the bound fraction of the conjugate was quantified by measuring the intensity of the emitted light. Calibration curves corresponding to analyte concentrations ranging from 0.5 ng mL-1 to 50 μg mL-1 (2.25 nM-225 μM) were obtained. A lowered detection limit by 2 orders of magnitude was obtained when detection was done in discontinuous mode and the chemiluminescence light was conducted inside the photomultiplier tube by an optical fiber bundle, thus yielding a dynamic range of 5 pg mL-1-100 ng mL-1 (22.5 pM-450 nM).
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| 4. |
- Ye, L., et al.
(författare)
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Towards the development of molecularly imprinted artificial receptors for the screening of estrogenic chemicals
- 2001
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Ingår i: Analyst. - : Royal Society of Chemistry (RSC). - 0003-2654. ; 126:6, s. 760-765
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Tidskriftsartikel (refereegranskat)abstract
- Molecularly imprinted polymers are prepared using various steroid compounds as the templates. The imprinted polymers can selectively re-bind the original print molecules, which leads to versatile potential applications. The feasibility of using these artificial receptors to replace their biological counterparts for preliminary screening of a chemical library is demonstrated. A steroid library composed of 22 closely related compounds is screened with an estrogen specific polymer. The print molecule is identified with accuracy and structural similarities of other members are correlated with normalized retention indices. Molecularly imprinted artificial receptors are envisioned as being useful for screening purposes in drug discovery or for identifying endocrine-disrupting chemicals.
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| 5. |
- Ye, Q, et al.
(författare)
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A novel pattern of pp65-positive cytomegalic endothelial cells circulating in peripheral blood from a renal transplant recipient
- 2004
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Ingår i: Acta Histochemica. - : Elsevier BV. - 0065-1281. ; 106:2, s. 107-110
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Tidskriftsartikel (refereegranskat)abstract
- The present study reports a novel pattern of cytomegalic endothelial cells (CEC) in peripheral blood from a female renal transplant recipient infected with human cytomegalovirus (HCMV), which has not been reported previously. Localization of specific early antigen of HCMV, pp65 antigen, was examined by immunohistochemistry. Staining of an endothelial cell marker (CD34) was used to characterize endothelial cells. It is demonstrated that many leukocytes surrounded and adhered to a protein-like material, in which pp65-positive CEC were detected. The composition and function of this protein-like material are yet unknown. The patient tacked clinical symptoms of HCMV disease. Furthermore, similar localization patterns were found in other renal. transplant recipients suffering from HCMV infections as determined by real-time PCR to detect HCMV DNA in blood. These patients showed no or only minor clinical symptoms of HCMV infection. It is suggested that these novel Localization patterns of CEC may play a role in the host defense in patients infected with HCMV, but the exact relation between HCMV infection and CEC formation needs further investigation. (C) 2004 Elsevier GmbH. All rights reserved.
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| 6. |
- Zhang, XY, et al.
(författare)
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Expression pattern of apolipoprotein M during mouse and human embryogenesis
- 2004
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Ingår i: Acta Histochemica. - : Elsevier BV. - 0065-1281. ; 106:2, s. 123-128
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Tidskriftsartikel (refereegranskat)abstract
- Apolipoprotein M (apoM) is a recently discovered human apolipoprotein predominantly present in high-density lipoprotein (HDL), and in minor proportions in triglyceride-rich lipoprotein (TGRLP) and low-density lipoprotein (LDL). The gene encoding apoM is present in all mammalian genomes. The identity of the apoM gene of human, rat and mouse is over 80%. However, the (patho)physiological functions of apoM are unknown yet. In the present study, we investigated apoM expression patterns during mouse and human embryogenesis. ApoM transcripts were detectable in mouse embryos from day 7.5 to day 18.5. ApoM was expressed at low levels at day 7.5, its expression increased significantly at day 9.7, decreased at day 10.5, and then increased continually up to day 18.5. ApoM-positive cells appeared mainly in liver of day-12 embryos as detected by in situ hybridization. In day-15 embryos, apoM was expressed in both liver and kidney. During human embryogenesis, apoM was mainly expressed in liver and kidney and little was found in small intestine as determined by mRNA array of human fetal. normal tissues. ApoM was also detected in stomach and skeletal muscle in early stages of embryogenesis (3-5 months). (C) 2004 Elsevier GmbH. All rights reserved.
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| 7. |
- Zhang, XY, et al.
(författare)
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Specific tissue expression and cellular localization of human apolipoprotein M as determined by in situ hybridization
- 2003
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Ingår i: Acta Histochemica. - : Elsevier BV. - 0065-1281. ; 105:1, s. 67-72
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Tidskriftsartikel (refereegranskat)abstract
- Apolipoprotein M (apoM) is a recently discovered human apolipoprotein predominantly present in high-density lipoprotein (HDL), and in minor proportion in triglyceride-rich lipoprotein (TGRLP) and low-density lipoprotein (LDL). The gene coding for apoM has been detected in all mammal genomes. The function of apoM is unknown yet. In the present study, we demonstrated that apoM is exclusively expressed in a strong manner in adult liver and kidney, and is expressed weakly in fetal liver and kidney as detected with human multiple tissue expression array. Both immumohistochemical staining and apoM mRNA in situ hybridization demonstrated that apoM was exclusively expressed in hepatocytes in human liver and in tubular epithelial cells in human kidney. The present study helps to elucidate the pathophysiological functions of apoM in vivo.
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