| 1. |
|
|
| 2. |
- Surugiu, Ioana, et al.
(författare)
-
Chemiluminescence imaging ELISA using an imprinted polymer as the recognition element instead of an antibody
- 2001
-
Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 73:3, s. 487-491
-
Tidskriftsartikel (refereegranskat)abstract
- An imaging assay analogous to competitive enzyme immunoassays has been developed using a molecularly imprinted polymer instead of an antibody. The antigen 2,4-dichlorophenoxyacetic acid (2,4-D) was labeled with tobacco peroxidase, and the chemiluminescence reaction of luminol was used for detection. Microtiter plates (96 or 384 wells) were coated with polymer microspheres imprinted with 2,4-D, which were fixed in place by using poly(vinyl alcohol) as glue. In a competitive mode, the analyte-peroxidase conjugate was incubated with the free analyte in the microtiter plate, after which the bound fraction of the conjugate was quantified. After addition of the chemiluminescent substrates, light emission was measured in a high-throughput imaging format with a CCD camera. Calibration curves corresponding to analyte concentrations ranging from 0.01 to 100 μg/mL were obtained.
|
|
| 3. |
- Ye, L., et al.
(författare)
-
Towards the development of molecularly imprinted artificial receptors for the screening of estrogenic chemicals
- 2001
-
Ingår i: Analyst. - : Royal Society of Chemistry (RSC). - 0003-2654. ; 126:6, s. 760-765
-
Tidskriftsartikel (refereegranskat)abstract
- Molecularly imprinted polymers are prepared using various steroid compounds as the templates. The imprinted polymers can selectively re-bind the original print molecules, which leads to versatile potential applications. The feasibility of using these artificial receptors to replace their biological counterparts for preliminary screening of a chemical library is demonstrated. A steroid library composed of 22 closely related compounds is screened with an estrogen specific polymer. The print molecule is identified with accuracy and structural similarities of other members are correlated with normalized retention indices. Molecularly imprinted artificial receptors are envisioned as being useful for screening purposes in drug discovery or for identifying endocrine-disrupting chemicals.
|
|
