| 1. |
- Awasthi, S. K., et al.
(författare)
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Sequential presence of heavy metal resistant fungal communities influenced by biochar amendment in the poultry manure composting process
- 2021
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Ingår i: Journal of Cleaner Production. - : Elsevier. - 0959-6526 .- 1879-1786. ; 291
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Tidskriftsartikel (refereegranskat)abstract
- In this study, we investigated the influence of coconut shell biochar (CSB) on heavy metal resistance fungi (HMRF) during poultry manure (PM) composting by 18 S rDNA Internal Transcribed Spacer Amplicon Sequencing analysis. Five different concentrations of CSB (2.5%, 5%, 7.5%, and 10% dry weights basis) were applied with a mixture of PM and wheat straw (5:1 ratio dry weight basis) and without CSB (CK) was used as control. The results showed that sequence number rose along with increasing CSB concentration but total relative abundance (RA) of HMRF decreased 56.33%, 74.65% in T4 and T5, respectively. However, greater RA of HMRF was found in T1 or without biochar applied treatment. The phylum of Basidiomycota was the dominant fungal community accounting for 61.14%, 6.16%, 32.18%, 74.65%, and 73.73% from T1 to T5 of the total fungi abundance, with wide presence of the Wallemiomycetes and Eurotiomycetes classes. The Wallemia and Aspergillus were the richest genus and species. Wallemia_sebi, Altemaria_alternata and Aspergillus_amoenus were detected having greater abundance among all treatments. Besides this, the network correlation pattern confirmed that the relative greater percentage of correlation among dominant HMRF community with bio-available HM and other physicochemical factors increased with the addition of biochar. There was reasonable infer that the biochar amendment in composting could constitute favorable habitat for an active fungal population.
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| 2. |
- Duan, Y., et al.
(författare)
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Evaluating the impact of bamboo biochar on the fungal community succession during chicken manure composting
- 2019
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Ingår i: Bioresource Technology. - : Elsevier Ltd. - 0960-8524 .- 1873-2976. ; 272, s. 308-314
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Tidskriftsartikel (refereegranskat)abstract
- The objective of this study was to investigate the fungal community succession and variations in chicken manure (CM) compost with different concentration of bamboo biochar (BB) as additive via the using of metagenomics method. The consequent obviously revealed that Chytridiomycota, Mucoromycota, Ascomycota and Basidiomycota were the dominant phylum, while Batrachochytrium, Funneliformis, Mucor, Phizophagus and Pyronema were the pre-dominant genera in each treatment. Redundancy analyses indicated that higher dosage of biochar applied treatments has significant correlation between fungal communities and environmental factors. The diversity of fungal community was analogous but the relative abundance (RA) was inconsistent among the all treatments. In addition, the principal component analysis was also confirmed that T5 and T6 treatments have considerably correlation than other treatments. However, the mean value of RA remained maximum in higher dosage of biochar blended treatments. Ultimately, the RA of different fungal genus and species were influenced in CM compost by the BB amendment.
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| 3. |
- Duan, Y M, et al.
(författare)
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Biochar regulates bacterial-fungal diversity and associated enzymatic activity during sheep manure composting
- 2022
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Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 346
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Tidskriftsartikel (refereegranskat)abstract
- Aimed to evaluate the coexistence of bacterial and fungal diversity and their correlation with enzymatic activity in response to biochar. This study performed aerobic composting based on typical agricultural wastes of sheep manure with additive apple tree branch biochar at distinct concentration (0, 2.5, 5, 7.5, 10 and 12.5% corresponding from T1 to T6). The result demonstrated that appropriate amendment of biochar enriched bacterial diversity (1646-1686 OTUs) but interestingly decreased fungal diversity (542-630 OTUs) compared to control (1444 and 682 OTUs). Biochar addition enhanced all enzymatic activities and its correlation with bacterial was more complex than fungal community (786 and 359 connect edges). The dominant microbes comprised of Firmicutes (45.2-35.2%), Proteobacteria (14.0-17.5%), Basidiomycota (32.4-49.5%) and Ascomycota (11.3-37.5%) among all the treatments. Overall, biochar regulates the composting microenvironment by influencing the microbial diversity and associated enzymatic activities.
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| 4. |
- Mukesh Kumar, Awasthi, et al.
(författare)
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Biotechnological strategies for bio-transforming biosolid into resources toward circular bio-economy : A review
- 2022
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Ingår i: Renewable & sustainable energy reviews. - : Elsevier. - 1364-0321 .- 1879-0690. ; 156
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Forskningsöversikt (refereegranskat)abstract
- Biosolids are the biological organic matter extracted from various treatment processes of wastewater which are considered as a rich source of energy and nutrients. The most commonly used method for the disposal of biosolids is landfilling. But this causes the loss of valuable nutrients and creates environmental issues. Circular economy approaches provide a better way for utilization these resources in a sustainable manner. This allows maximum utilization of resources and many natural resources can be preserved and utilized for future generations. The present review provides a comprehensive illustration of biotechnological approaches for the utilization of biosolids. Various process strategies for the utilization of biosolids for the production of energy, fuels and valueadded products are discussed. The utilization of this rich organic matter under circular economy has also been described in detail.
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| 5. |
- Awasthi, M, et al.
(författare)
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Emerging applications of biochar : Improving pig manure composting and attenuation of heavy metal mobility in mature compost
- 2020
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Ingår i: Journal of Hazardous Materials. - : Elsevier B.V.. - 0304-3894 .- 1873-3336. ; 389
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Tidskriftsartikel (refereegranskat)abstract
- This study evaluated the effect of integrated bacterial culture and biochar on heavy metal (HM) stabilization and microbial activity during pig manure composting. High-throughput sequencing was carried out on six treatments, namely T1-T6, where T2 was single application of bacteria culture (C), T3 and T5 were supplemented with 12 % wood (WB) and wheat-straw biochar (WSB), respectively, and T4 and T6 had a combination of bacterial consortium mixed with biochar (12 % WB and 12 % WSB, respectively). T1 was used as control for the comparison. The results show that the populations of bacterial phyla were significantly greater in T6 and T4. The predominate phylum were Proteobacteria (56.22 %), Bacteroidetes (35.40 %), and Firmicutes (8.38 %), and the dominant genera were Marinimicrobium (53.14 %), Moheibacter (35.22 %), and Erysipelothrix (5.02 %). Additionally, the correlation analysis revealed the significance of T6, as the interaction of biochar and bacterial culture influenced the HM adsorption efficiency and microbial dynamics during composting. Overall, the integrated bacterial culture and biochar application promoted the immobilization of HMs (Cu and Zn) owing to improved adsorption, and enhanced the abundance and selectivity of the bacterial community to promote degradation and improving the safety and quality of the final compost product. © 2020 Elsevier B.V.
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| 6. |
- Awasthi, S. K., et al.
(författare)
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Can biochar regulate the fate of heavy metals (Cu and Zn) resistant bacteria community during the poultry manure composting?
- 2021
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Ingår i: Journal of Hazardous Materials. - : Elsevier. - 0304-3894 .- 1873-3336. ; 406
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Tidskriftsartikel (refereegranskat)abstract
- In this study, the influence of coconut shell biochar addition (CSB) on heavy metals (Cu and Zn) resistance bacterial fate and there correlation with physicochemical parameters were evaluated during poultry manure composting. High-throughput sequencing was carried out on five treatments, namely T1−T5, where T2 to T5 were supplemented with 2.5%, 5%, 7.5% and 10% CSB, while T1 was used as control for the comparison. The results of HMRB indicated that the relative abundance of major potential bacterial host altered were Firmicutes (52.88–14.32%), Actinobacteria (35.20–4.99%), Bacteroidetes (0.05–15.07%) and Proteobacteria (0.01–20.28%) with elevated biochar concentration (0%−10%). Beta and alpha diversity as well as network analysis illustrated composting micro-environmental ecology with exogenous additive biochar to remarkably affect the dominant resistant bacterial community distribution by adjusting the interacting between driving environmental parameters with potential host bacterial in composting. Ultimately, the amendment of 7.5% CSB into poultry manure composting was able to significantly reduce the HMRB abundance, improve the composting efficiency and end product quality.
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| 7. |
- Awasthi, S K, et al.
(författare)
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Multi-criteria research lines on livestock manure biorefinery development towards a circular economy : From the perspective of a life cycle assessment and business models strategies
- 2022
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Ingår i: Journal of Cleaner Production. - : Elsevier. - 0959-6526 .- 1879-1786. ; 341
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Tidskriftsartikel (refereegranskat)abstract
- Livestock manure (LSM) is a profitable waste if handled sensibly, but simultaneously it imposes several environmental and health impacts if managed improperly. Several approaches have been adopted globally to cartel the problem associated with LSM management and recovery of value-added products, still, technological innovation needs further upgradation in consideration with the environment, energy, and economy. This review delivered a vibrant portrait of manure management, which includes, bioenergy generation and resource recovery strategies, their current scenario, opportunities, challenges, and prospects for future researches along with global regulations and policies. Several bioenergy generation and nutrient recoveries technologies have been discussed in details, still, the major glitches allied with these technologies are its high establishment costs, operational costs, manure assortment, and digestate handling. This review also discussed the techno-economic assessment (TEA) and life cycle assessment (LCA) of LSM management operation in the context of their economical and environmental sustainability. Still, extensive researches needed to build an efficient manure management framework to advance the integrated bioenergy production, nutrients recycling, and digestate utilization with least environmental impacts and maximal economical gain, which has critically discussed in the current review.
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| 8. |
- Dhakal, Hom N, et al.
(författare)
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Falling weight impact response of jute/methacrylated soybean oil bio-composites under low velocity impact loading
- 2014
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Ingår i: Composites Science And Technology. - : Pergamon. - 0266-3538 .- 1879-1050. ; 92, s. 134-141
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Tidskriftsartikel (refereegranskat)abstract
- In this study, impact performance of bio-composites fabricated from jute/methacrylated soybean oil (MSO) subjected to low-velocity impact loading is presented. The composite laminates were fabricated using compression moulding technique and their thickness and weave architectures effect on the impact response were investigated and the experimental observations are reported. From the results obtained, it was observed that fibre orientation and thickness variation have a significant influence on the impact resistance of jute/MSO composite material. The results show that the total absorbed energy and maximum peak load increase linearly with an increase in the thickness. Among the composite samples investigated where thickness comprised of: 1, 1.5, 2, 2.5 and 3 mm, a composite reinforced with 46 yarns per 10 cm weft and 50 warp (W2-3 mm thick) is found to have highest resistance to impact damage compared to 32 and 15 yarn per 10 cm weft samples. This was attributed to the improved fibre/matrix interface as a result of surface treatment of jute fibres and the fibre architectures effect which create the cross-over points which act as stress distributors.
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| 9. |
- Duan, Y., et al.
(författare)
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Dynamics of fungal diversity and interactions with environmental elements in response to wheat straw biochar amended poultry manure composting
- 2019
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Ingår i: Bioresource Technology. - : Elsevier Ltd. - 0960-8524 .- 1873-2976. ; , s. 410-417
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Tidskriftsartikel (refereegranskat)abstract
- The fungal dynamics and its correlation with physicochemical and gaseous emission were investigated using metagenomics and Heat map illustrator (HEMI). Five different concentrations of wheat straw biochar (WSB) were applied to poultry manure (PM) and composted for 50 days; those without the WSB treatment were used as a control. The results revealed the dominant phyla to be Chytridiomycota, Mucoromycota, Ascomycota and Basidiomycota, while Batrachochytrium, Rhizophagus, Mucor, and Puccinia were the superior genera. In particular, the diversity of Chytridiomycota and Ascomycota was more abundant among all of the treatments. Overall, the diversity of the fungal species was correspondent, but relative abundance varied significantly among all of the composts. Principle Coordinate Analysis (PCoA) and Non-Metric Multi- Dimensional Scaling (NMDS) indicated that different concentrations of WSB applied treatments have significantly distinct fungal communities. In addition, correlation analyses of fungal interactions with environmental elements via HEMI also indicate a clear difference among the treatments. Ultimately, the relative abundance of fungal composition significantly influenced the PM compost treated by the WSB.
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| 10. |
- Duan, Y., et al.
(författare)
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Organic solid waste biorefinery : Sustainable strategy for emerging circular bioeconomy in China
- 2020
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Ingår i: Industrial crops and products (Print). - : Elsevier B.V.. - 0926-6690 .- 1872-633X. ; 153
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Tidskriftsartikel (refereegranskat)abstract
- Biorefinery perceived as impeccable encouraging perspective of generating multiple bio-products from organic solid waste through integration of various relevant techniques. With the improved attractiveness of circular bio-economy as a greatly potential approach in view of social soaring energy demands and biodiversity conservation, biorefinery contributed as sustainable strategic mechanism for emerging circular bio-economy. Present study outlined a comprehensive review of the status for organic solid waste derived from agriculture, industry and urban toward to available biorefinery approach. In addition, evaluated techno-economic assessment and proposed the current bottleneck challenges and future perspective for sustainability of circular bio-economy in China. The results demonstrated that the diversity kinds of solid waste played an essential role in implement of biorefinery in circular bio-economy. Achieving sustainable circular bio-economy requires coordinated development of economic, environment and social. There is still a great gap between actual performance with expectation of biorefinery in bio-economy, comprehensive investigations and depth exploration were critical for sustainability.
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