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Sökning: WFRF:(McLean Catriona) > (2009)

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  • Grünblatt, Edna, et al. (författare)
  • Tryptophan is a marker of human postmortem brain tissue quality
  • 2009
  • Ingår i: Journal of Neurochemistry. - 0022-3042 .- 1471-4159. ; 110:5, s. 1400-1408
  • Tidskriftsartikel (refereegranskat)abstract
    • Postmortem human brain tissue is widely used in neuroscience research, but use of tissue originating from different brain bank centers is considered inaccurate because of possible heterogeneity in sample quality. There is thus a need for well-characterized markers to assess the quality of postmortem brain tissue. Toward this aim, we determined tryptophan (TRP) concentrations, phosphofructokinase-1 and glutamate decarboxylase activities in 119 brain tissue samples. These neurochemical parameters were tested in samples from autopsied individuals, including control and pathological cases provided by 10 different brain bank centers. Parameters were assessed for correlation with agonal state, postmortem interval, age and gender, brain region, preservation and freezing methods, storage conditions and storage time, RNA integrity, and tissue pH value. TRP concentrations were elevated significantly (p = 0.045) with increased postmortem interval; which might indicate increased protein degradation. Therefore, TRP concentration might be one useful and convenient marker for estimating the quality of human postmortem brain tissue.
  • Monoranu, Camelia Maria, et al. (författare)
  • pH measurement as quality control on human postmortem brain tissue : A Study of the BrainNet Europe Consortium.
  • 2009
  • Ingår i: Neuropathology and Applied Neurobiology. - 0305-1846 .- 1365-2990. ; 35:3, s. 329-337
  • Tidskriftsartikel (refereegranskat)abstract
    • Aims: Most brain diseases are complex entities. Although animal models or cell culture experiments mimic some disease aspects, human postmortem brain tissue remains essential to advance our understanding of brain diseases using biochemical and molecular techniques. Postmortem artifacts must be properly understood, standardized, and either eliminated or factored into such experiments. Here we examine the influence of several pre- and postmortem factors on pH, and discuss the role of pH as a biochemical marker for brain tissue quality. Methods: We assessed brain tissue pH in 339 samples from 116 brains provided by 8 different European and 2 Australian brain bank centres. We correlated brain pH with tissue source, postmortem delay, age, gender, freezing method, storage duration, agonal state, or and brain ischaemia. Results: Our results revealed that only prolonged agonal state and ischaemic brain damage influenced brain tissue pH next to repeated freeze/thaw cycles. Conclusions: pH measurement in brain tissue is a good indicator of premortem events in brain tissue and it signals limitations for postmortem investigations.
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