Variability of the glycoprotein G gene in clinical isolates of herpes simplex virus type 1.

• Glycoprotein G (gG) of herpes simplex virus type 1 (HSV-1) has been used as a prototype antigen for HSV-1 type-specific serodiagnosis, but data on the sequence variability of the gene coding for this protein in wild-type strains are lacking. In this study, direct DNA sequencing of the gG-1 genes from PCR products was performed with clinical HSV-1 isolates from 11 subjects as well as with strains Syn 17(+), F, and KOS 321. The reference strains Syn 17(+) and F showed a high degree of conservation, while KOS 321 carried 13 missense mutations and, in addition, 12 silent mutations. Three clinical isolates showed mutations leading to amino acid alterations: one had a mutation of K(122) to N, which is a gG-1-to-gG-2 alteration; another contained all mutations which were observed in KOS 321 except two silent mutations; and the third isolate carried five missense mutations. Two clinical isolates as well as strain KOS 321 showed a mutation (F(111)-->V) within the epitope of a gG-1-reactive monoclonal antibody (MAb). When all viruses were tested for reactivity with the anti-gG-1 MAb, the three strains with the F(111)-->V mutation were found to be unreactive. Furthermore, gG-1 antibodies purified from sera from the two patients carrying strains mutated in this epitope were less reactive when they were tested by an HSV-1-infected-cell assay. Therefore, our finding that the sequence variability of the gG-1 gene also affects B-cell epitope regions of this protein in clinical isolates may have consequences for the use of this protein as a type-specific antigen for serodiagnosis.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)

Nyckelord

Adult

Aged

Amino Acid Sequence

Amino Acid Substitution

Animals

Antibodies

Monoclonal

Antibodies

Viral

immunology

Antigens

Surface

analysis

immunology

Cells

Cultured

Cercopithecus aethiops

DNA Mutational Analysis

DNA

Viral

analysis

Enzyme-Linked Immunosorbent Assay

Epitopes

immunology

Female

Genetic Variation

Herpes Simplex

genetics

immunology

Herpesvirus 1

Human

genetics

immunology

Humans

Immunoglobulin G

immunology

Kidney

cytology

Male

Middle Aged

Molecular Sequence Data

Polymerase Chain Reaction

Sensitivity and Specificity

Serologic Tests

Viral Envelope Proteins

genetics

immunology

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)