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Variability of the ...
Variability of the glycoprotein G gene in clinical isolates of herpes simplex virus type 1.
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- Rekabdar, Elham, 1971 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk virologi,Institute of Laboratory Medicine, Dept of Clinical Virology
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- Tunbäck, Petra, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för dermatologi och venereologi,Institute of Selected Clinical Sciences, Department of Dermatology and Venereology
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- Liljeqvist, Jan-Åke, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk virologi,Institute of Laboratory Medicine, Dept of Clinical Virology
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visa fler...
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- Bergström, Tomas, 1950 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk virologi,Institute of Laboratory Medicine, Dept of Clinical Virology
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visa färre...
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(creator_code:org_t)
- 1999
- 1999
- Engelska.
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Ingår i: Clinical and diagnostic laboratory immunology. - 1071-412X. ; 6:6, s. 826-31
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Glycoprotein G (gG) of herpes simplex virus type 1 (HSV-1) has been used as a prototype antigen for HSV-1 type-specific serodiagnosis, but data on the sequence variability of the gene coding for this protein in wild-type strains are lacking. In this study, direct DNA sequencing of the gG-1 genes from PCR products was performed with clinical HSV-1 isolates from 11 subjects as well as with strains Syn 17(+), F, and KOS 321. The reference strains Syn 17(+) and F showed a high degree of conservation, while KOS 321 carried 13 missense mutations and, in addition, 12 silent mutations. Three clinical isolates showed mutations leading to amino acid alterations: one had a mutation of K(122) to N, which is a gG-1-to-gG-2 alteration; another contained all mutations which were observed in KOS 321 except two silent mutations; and the third isolate carried five missense mutations. Two clinical isolates as well as strain KOS 321 showed a mutation (F(111)-->V) within the epitope of a gG-1-reactive monoclonal antibody (MAb). When all viruses were tested for reactivity with the anti-gG-1 MAb, the three strains with the F(111)-->V mutation were found to be unreactive. Furthermore, gG-1 antibodies purified from sera from the two patients carrying strains mutated in this epitope were less reactive when they were tested by an HSV-1-infected-cell assay. Therefore, our finding that the sequence variability of the gG-1 gene also affects B-cell epitope regions of this protein in clinical isolates may have consequences for the use of this protein as a type-specific antigen for serodiagnosis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Nyckelord
- Adult
- Aged
- Amino Acid Sequence
- Amino Acid Substitution
- Animals
- Antibodies
- Monoclonal
- Antibodies
- Viral
- immunology
- Antigens
- Surface
- analysis
- immunology
- Cells
- Cultured
- Cercopithecus aethiops
- DNA Mutational Analysis
- DNA
- Viral
- analysis
- Enzyme-Linked Immunosorbent Assay
- Epitopes
- immunology
- Female
- Genetic Variation
- Herpes Simplex
- genetics
- immunology
- Herpesvirus 1
- Human
- genetics
- immunology
- Humans
- Immunoglobulin G
- immunology
- Kidney
- cytology
- Male
- Middle Aged
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Serologic Tests
- Viral Envelope Proteins
- genetics
- immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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