| 1. |
- Andreyev, HJN, et al.
(författare)
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Kirsten ras mutations in patients with colorectal cancer : The 'RASCAL II' study
- 2001
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Ingår i: British Journal of Cancer. - : Springer Science and Business Media LLC. - 0007-0920 .- 1532-1827. ; 85:5, s. 692-696
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Tidskriftsartikel (refereegranskat)abstract
- Researchers worldwide with information about the Kirsten ras (Ki-ras) tumour genotype and outcome of patients with colorectal cancer were invited to provide that data in a schematized format for inclusion in a collaborative database called RASCAL (The Kirsten ras incolorectal-cancer collaborative group). Our results from 2721 such patients have been presented previously and for the first time in any common cancer, showed conclusively that different gene mutations have different impacts on outcome, even when the mutations occur at the same site on the genome. To explore the effect of Ki-ras mutations at different stages of colorectal cancer, more patients were recruited to the database, which was reanalysed when information on 4268 patients from 42 centres in 21 countries had been entered. After predetermined exclusion criteria were applied, data on 3439 patients were entered into a multivariate analysis. This found that of the 12 possible mutations on codons 12 and 13 of Kirsten ras, only one mutation on codon 12, glycine to valine, found in 8.6% of all patients, had a statistically significant impact on failure-free survival (P=0.004, HR 1.3) and overall survival (P=0.008, HR 1.29). This mutation appeared to have a greater impact on outcome in Dukes' C cancers (failure-free survival, P=0.008, HR 1.5, overall survival P=0.02, HR 1.45) than in Dukes' B tumours (failure-free survival, P=0.46, HR 1.12, overall survival P=0.36, HR 1.15). Ki-ras mutations may occur early in the development of pre-cancerous adenomas in the colon and rectum. However, this collaborative study suggests that not only is the presence of a codon 12 glycine to valine mutation important for cancer progression but also that it may predispose to more aggressive biological behaviour in patients with advanced colorectal cancer. ⌐ 2001 Cancer Research Campaign.
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| 2. |
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| 3. |
- Chen, Yun, 1966, et al.
(författare)
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Neonatal losartan treatment suppresses renal expression of molecules involved in cell-cell and cell-matrix interactions
- 2004
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Ingår i: Journal of the American Society of Nephrology. - : Lippincott Williams & Wilkins. - 1046-6673 .- 1533-3450. ; 15:5, s. 1232-43
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Tidskriftsartikel (refereegranskat)abstract
- Lack of neonatal angiotensin II type 1 receptor (AT(1)) stimulation produces renal abnormalities characterized by papillary atrophy and impaired urinary concentrating ability, but the mechanisms involved are still unclear. DNA microarray was used to identify genes that are differentially expressed in renal medulla in response to neonatal treatment with AT(1) receptor antagonist losartan (30 mg/kg per d), which commenced within 24 h after birth. The data showed that losartan treatment for 48 h downregulated 68 genes, approximately 30% of which encode various components of cytoskeleton and cytoskeleton-associated proteins, extracellular matrix, and enzymes involved in extracellular matrix maturation or turnover. With the use of immunohistochemistry and Western immunoblot, the microarray data were confirmed and it was demonstrated that losartan suppressed renal expression of syndecan 2, alpha-smooth muscle actin, MHC class II, and leukocyte type 12-lipoxygenase by day 4. In addition, losartan inhibited medullary expression of integrin alpha6 and caused relocalization of integrins alpha6 and alpha3. Moreover, losartan inhibited cell proliferation in medullary tubules by day 9, as detected by Ki-67 immunostaining. This study provides new data supporting the contention that a lack of AT(1) receptor stimulation results in abnormal matrix assembly, disturbed cell-cell and cell-matrix interactions, and subsequent abnormal tubular maturation. Moreover, regulation of the expression of leukocyte type 12-lipoxygenase and alpha-smooth muscle actin by the renin-angiotensin system in the immature kidney adds new knowledge toward the understanding of renal vascular development.
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| 4. |
- Feng, Q., et al.
(författare)
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A new method for in-situ non-contact roughness measurement of large rock fracture surfaces
- 2003
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Ingår i: Rock Mechanics and Rock Engineering. - : Springer Science and Business Media LLC. - 0723-2632 .- 1434-453X. ; 36:1, s. 3-25
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents a new method for in-situ non-contact measurements of fracture roughness by using a total station (TS). The TS is a non-reflector geodetic instrument usually used for measuring control points in surveying and mapping. By using a special-developed program, the TS can be used as a point-sensor laser scanner to scan a defined area of the fracture surface automatically, in field or in laboratory, at a distance away from the target surface. A large fracture surface can be automatically scanned with a constant interval of the sampling points, both within a defined area or along a cross-section of the exposed rock face. To quantify fracture roughness at different scales and obtain different densities of the scanned points, the point interval can be selected with the minimum interval of I rum. A local Cartesian co-ordinate system needs to be established first by the TS in front of the target rock face to define the true North or link the measurements to a known spatial co-ordinate system for both quantitative and spatial analysis of fracture roughness. To validate the method, fracture roughness data recorded with a non-reflector TS was compared with the data captured by a high-accuracy 3D-laser scanner. Results of this study revealed that both primary roughness and waviness of fracture surfaces can be quantified by the TS in the same accuracy level as that of the high accuracy laser scanner. Roughness of a natural fracture surface can be sampled without physical contact in a maximum distance of tens of meters from the rock faces.
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| 5. |
- Hong, Feng, et al.
(författare)
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Rapid and convenient determination of oxalic acid employing a novel oxalate biosensor based on oxalate oxidase and SIRE technology
- 2003
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Ingår i: Biosensors & Bioelectronics. - 1873-4235. ; 18:9, s. 1173-1181
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Tidskriftsartikel (refereegranskat)abstract
- A new method for rapid determination of oxalic acid was developed using oxalate oxidase and a biosensor based on SIRE (sensors based on injection of the recognition element) technology. The method was selective, simple, fast, and cheap compared with other present detection systems for oxalate. The total analysis time for each assay was 2-9 min. A linear range was observed between 0 and 5 mM when the reaction conditions were 30 degreesC and 60 s. The linear range and upper limit for concentration determination could be increased to 25 mM by shortening the reaction time. The lower limit of detection in standard solutions, 20 muM, could be achieved by means of modification of the reaction conditions, namely increasing the temperature and the reaction time. The biosensor method was compared with a conventional commercially available colorimetric method with respect to the determination of oxalic acid in urine samples. The urine oxalic acid concentrations determined with the biosensor method correlated well (R = 0.952) with the colorimetric method. (C) 2002 Elsevier Science B.V. All rights reserved.
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| 6. |
- Wang, Lihui, et al.
(författare)
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Architecture Design for Distributed Process Planning
- 2003
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Ingår i: Journal of manufacturing systems. - : Society of Manufacturing Engineers, North American Manufacturing Research Institution. - 0278-6125 .- 1878-6642. ; 22:2, s. 99-115
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Tidskriftsartikel (refereegranskat)abstract
- Today's machining shop floors, characterized by a large variety of products in small batch sizes, require dynamic process planning capabilities that are responsive and adaptive to the rapid changes of production capacity and functionality. To meet the requirement, this research proposes a new methodology for dynamic and distributed process planning. The primary focus of this paper is on the architecture of a new approach using function blocks. The secondary focus is given to the other supporting technologies-machining features and agents. Different from conventional methods, this approach uses a two-layer structure-supervisory planning and operation planning. It is expected that the new architecture can improve the system performance in a dynamic environment.
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| 7. |
- Zhang, X. F., et al.
(författare)
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catena-Poly (1,10-phenanthroline-kappa N-2,N ')manganese(II) -mu-L-tartrato-kappa O-4(1),O-2 : O-3,O-4 hexahydrate
- 2003
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Ingår i: Acta Crystallographica Section C. - 0108-2701 .- 1600-5759. ; 59, s. M402-M404
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Tidskriftsartikel (refereegranskat)abstract
- The title compound, {[Mn(C4H4O6)(C12H8N2)] . 6H(2)O}(n), has a linear chain structure containing monomeric [ Mn( C4H4O6)( C12H8N2)] repeat units. Each manganese ion is six-coordinate, with the two phenanthroline N atoms [Mn - N = 2.229 (2) and 2.235 (2) Angstrom] and four O atoms from two tartrate anions [Mn - O-COO = 2.1252 (19) and 2.1310 (19) Angstrom, and Mn - O-OH = 2.2404 (19) and 2.2424 (19) Angstrom] forming a seriously distorted octahedral coordination environment. Six water molecules exist outside every repeat unit as solvate molecules. Extensive hydrogen-bonding interactions and pi-pi stacking of the phenanthroline moieties exist between the chains.
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