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Träfflista för sökning "WFRF:(Fritzsche Joachim) srt2:(2018)"

Sökning: WFRF:(Fritzsche Joachim) > (2018)

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1.
  • Werner, Erik, et al. (författare)
  • Hairpins in the conformations of a confined polymer
  • 2018
  • Ingår i: Biomicrofluidics. - : AIP Publishing. - 1932-1058. ; 12:2
  • Tidskriftsartikel (refereegranskat)abstract
    • If a semiflexible polymer confined to a narrow channel bends around by 180°, the polymer is said to exhibit a hairpin. The equilibrium extension statistics of the confined polymer are well understood when hairpins are vanishingly rare or when they are plentiful. Here, we analyze the extension statistics in the intermediate situation via experiments with DNA coated by the protein RecA, which enhances the stiffness of the DNA molecule by approximately one order of magnitude. We find that the extension distribution is highly non-Gaussian, in good agreement with Monte-Carlo simulations of confined discrete wormlike chains. We develop a simple model that qualitatively explains the form of the extension distribution. The model shows that the tail of the distribution at short extensions is determined by conformations with one hairpin. © 2018 Author(s).
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2.
  • Westerlund, Fredrik, 1978, et al. (författare)
  • Fluorescence microscopy of nanochannel-confined DNA
  • 2018
  • Ingår i: Methods in Molecular Biology. - New York, NY : Springer New York. - 1064-3745 .- 1940-6029. ; 1665, s. 173-198
  • Bokkapitel (refereegranskat)abstract
    • Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.
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