| 21. |
- Kurth, W.S., Hospodarsky, G.B., Gurnett, D.A., Kaiser, M.L., Wahlund, J.E., Roux, A., Canu, P., Zarka, P. and Tokarev, Y.
(författare)
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An overview of observations by the Cassini radio and plasma wave investigation at Earth.
- 2001
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Ingår i: Journal of Geophysical Research. ; 106:A12, s. 30239-
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Tidskriftsartikel (refereegranskat)abstract
- On August 18, 1999, the Cassini spacecraft flew by Earth at an altitude of 1186 km on its way to Saturn. Although the flyby was performed exclusively to provide the spacecraft with sufficient velocity to get to Saturn, the radio and plasma wave science (R
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| 22. |
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| 23. |
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| 24. |
- Saarne, T, et al.
(författare)
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Cloning and characterisation of two IgE-binding proteins, homologous to tropomyosin and alpha-tubulin, from the mite Lepidoglyphus destructor
- 2003
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Ingår i: International archives of allergy and immunology. - : S. Karger AG. - 1018-2438 .- 1423-0097. ; 130:4, s. 258-265
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Tidskriftsartikel (refereegranskat)abstract
- <i>Background:</i> The dust mite <i>Lepidoglyphus destructor</i> is a major source of mite allergy in European rural environments, but it also causes allergy in urban populations around the world. We have previously cloned, sequenced and expressed several allergens from <i>L. destructor</i> (Lep d 2, Lep d 5, Lep d 7 and Lep d 13). The aim of this study was to identify and clone additional allergens from <i>L. destructor</i>, and to evaluate their IgE-binding reactivities. <i>Methods:</i> PCR and screening with sera from <i>L. destructor</i>-sensitised individuals were used to isolate new clones from a phage display <i>L. destructor</i> cDNA library. The complete coding sequences of the clones were determined and expressed as His<sub>6</sub>-tagged recombinant proteins in <i>Escherichia coli</i>. The recombinant proteins were analysed by SDS-PAGE, immunoblotting and mass spectrometry. <i>Results:</i> Two new clones, showing homology to tropomyosin and α-tubulin in several species, were isolated from the phage display <i>L. destructor</i> cDNA library. Due to its homology to group 10 dust mite allergens, the tropomyosin clone was named Lep d 10. The IgE-binding frequencies of the recombinant Lep d 10 and α-tubulin were 13% (18/136) and 12% (11/95), respectively, among subjects with IgE reactivity to mites and/or crustaceans. <i>Conclusions:</i> Two new allergens from <i>L. destructor</i> have been identified and can now be added to the repertoire of recombinant <i>L. destructor</i> allergens. In addition, both these allergens belong to highly conserved protein families and may be important for evaluation of allergenic cross-reactivity.
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| 25. |
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| 26. |
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| 27. |
- Spanbroek, R, et al.
(författare)
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Expanding expression of the 5-lipoxygenase pathway within the arterial wall during human atherogenesis
- 2003
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424. ; 100:3, s. 1238-1243
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Tidskriftsartikel (refereegranskat)abstract
- Oxidation products of low-density lipoproteins have been suggested to promote inflammation during atherogenesis, and reticulocyte-type 15-lipoxygenase has been implicated to mediate this oxidation. In addition, the 5-lipoxygenase cascade leads to formation of leukotrienes, which exhibit strong proinflammatory activities in cardiovascular tissues. Here, we studied both lipoxygenase pathways in human atherosclerosis. The 5-lipoxygenase pathway was abundantly expressed in arterial walls of patients afflicted with various lesion stages of atherosclerosis of the aorta and of coronary and carotid arteries. 5-lipoxygenase localized to macrophages, dendritic cells, foam cells, mast cells, and neutrophilic granulocytes, and the number of 5-lipoxygenase expressing cells markedly increased in advanced lesions. By contrast, reticulocyte-type 15-lipoxygenase was expressed at levels that were several orders of magnitude lower than 5-lipoxygenase in both normal and diseased arteries, and its expression could not be related to lesion pathology. Our data support a model of atherogenesis in which 5-lipoxygenase cascade-dependent inflammatory circuits consisting of several leukocyte lineages and arterial wall cells evolve within the blood vessel wall during critical stages of lesion development. They raise the possibility that antileukotriene drugs may be an effective treatment regimen in late-stage disease.
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