SwePub
Sök i SwePub databas

  Extended search

Träfflista för sökning "WFRF:(Tingström Anders) srt2:(1990-1994);srt2:(1990)"

Search: WFRF:(Tingström Anders) > (1990-1994) > (1990)

  • Result 1-2 of 2
Sort/group result
   
EnumerationReferenceCoverFind
1.
  •  
2.
  • Tingström, Anders, et al. (author)
  • C-CAM (cell-CAM 105) is an adhesive cell surface glycoprotein with homophilic binding properties
  • 1990
  • In: Journal of Cell Science. - 0021-9533. ; 96:1, s. 17-25
  • Journal article (peer-reviewed)abstract
    • C-CAM (Cell-CAM 105) is a cell surface glycoprotein that is involved in cell-cell adhesion of rat hepatocytes in vitro. To elucidate the adhesion mechanism the binding properties of purified C-CAM were investigated. Using proteins immobilized on nitrocellulose it was found that radiolabeled C-CAM bound to C-CAM but not to a variety of other proteins. Partitioning in Triton X-114 showed that C-CAM has hydrophobic properties. In accordance with this, C-CAM was effectively incorporated into phosphatidylcholine liposomes by dialysis from octylglucoside-containing solutions. The C-CAM-containing liposomes bound specifically to isolated hepatocytes. This binding was blocked by Fab fragments of anti-C-CAM antibodies. Furthermore, preincubation of hepatocytes with anti-C-CAM antibodies followed by washing of the cells blocked binding of C-CAM-containing liposomes. At increasing C-CAM contents in the reconstituted liposomes a marked self-aggregation of the liposomes occurred. This aggregation was blocked by Fab fragments of anti-C-CAM antibodies and by alkaline pH. After neutralization a rapid reaggregation occurred. Neither C-CAM binding to C-CAM immobilized on nitrocellulose nor C-CAM-liposome aggregation required calcium ions. Liposomes reconstituted with C-CAM-depleted membrane glycoproteins did not self-aggregate or bind to hepatocytes. Thus, it is concluded that C-CAM can bind specifically to C-CAM in a homophilic binding reaction that does not require calcium. Accordingly, C-CAM has the potential of directly mediating cell-cell adhesion via C-CAM-C-CAM binding between adjacent cells.
  •  
Skapa referenser, mejla, bekava och länka
  • Result 1-2 of 2
Type of publication
journal article (2)
Type of content
peer-reviewed (2)
Author/Editor
Tingström, Anders (2)
Rubin, Kristofer (1)
Olsson, L (1)
Blikstad, Ingrid (1)
Borg, T.K. (1)
Terracio, L (1)
show more...
Gullberg, Donald (1)
Thuresson, A C (1)
Aurivillius, Magnus (1)
Obrink, Björn (1)
show less...
University
Uppsala University (1)
Lund University (1)
Language
English (2)
Research subject (UKÄ/SCB)
Medical and Health Sciences (1)
Year

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view