| 1. |
- Geborek, Pierre, et al.
(author)
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Measurement of oxygen and carbon dioxide partial pressures in synovial fluid after tonometry
- 1988
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In: Clinical Physiology. - 1365-2281. ; 8:4, s. 427-432
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Journal article (peer-reviewed)abstract
- A commercially available gas analyser was used to measure tension of oxygen (PO2) and carbon dioxide (PCO2) in synovial fluid samples after tonometry. Measured values of PCO2 were close to the expected (median difference 0.2 kPa, range -0.4 to 0.4) within the analysed concentration range of 4-10 kPa. No consistent difference between measured and expected values of PO2 were found for oxygen in the range 3-11 kPa (median difference 0.1 kPa, range -0.3 to 1.2). For oxygen tensions below 3 kPa, however, the measured values invariably overestimated the actual PO2, the errors ranging from 0.3 to 1.9 kPa, median 1.1. The importance of proper handling of samples was investigated and storage for 1 h at 0 degrees C in plastic syringes resulted in elevation of the PO2 levels measured (range of elevation 0.2 to 3.6 kPa, median 1.15), whilst no significant differences were found when stored in glass syringes. Within the limits stated, commercially available gas analysers may thus be used to investigate these parameters related to local tissue metabolism in effusive joint conditions.
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| 2. |
- Peetre, Christina, et al.
(author)
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A tumor necrosis factor binding protein is present in human biological fluids
- 1988
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In: European Journal of Haematology. - : Wiley. - 0902-4441 .- 1600-0609. ; 41:5, s. 414-419
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Journal article (peer-reviewed)abstract
- Tumor necrosis factor (TNF) possesses both beneficial and toxic bioactivities. Mechanisms may operate to counteract harmful effects. We have identified a TNF binding protein (TNF-BP), which shows increased levels in serum and urine of patients on regular hemodialysis treatment (RDT). TNF-BP inhibited the specific binding of human recombinant TNF (rTNF) to its cell surface receptor. Results from gel chromatography demonstrated the presence in serum and urine of a macromolecule with an apparent molecular weight of 50,000, which formed a complex with rTNF. A 62-fold purification of TNF-BP from urine of patients on RDT was achieved by ion exchange chromatography and gel chromatography. Partially purified TNF-BP reduced the growth inhibitory effect of rTNF on a susceptible leukemia cell line. TNF-BP may act as a regulator of the biological activity of TNF and could have beneficial effects in certain inflammatory conditions.
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| 3. |
- Truedsson, L, et al.
(author)
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Protein HC-IgA complexes carry antibody activities
- 1988
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In: Scandinavian Journal of Immunology. - : Wiley. - 0300-9475 .- 1365-3083. ; 27:2, s. 201-208
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Journal article (peer-reviewed)abstract
- Polyclonal protein HC-IgA complexes (HC-IgA) were isolated from two different serum pools. Their hydrodynamic volumes were found to be slightly greater than that of monomeric IgA but less than that of dimeric IgA. Sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis of reduced and carboxymethylated complexes followed by immunoblotting showed that the complexes contained normal light and heavy Ig chains, and one polypeptide chain with Mr = 90,000, which carried both IgA alpha chain and protein HC epitopes. Enzyme-linked immunosorbent assays (ELISA) demonstrated that the isolated HC-IgA carried about 0.1 and 4%, respectively, of the antibody activities against one carbohydrate antigen (Yersinia enterocolitica serotype 0:3 lipopolysaccharide) and one protein antigen (rabbit IgG, i.e. antigen for rheumatoid factors) of the IgA populations of the two serum pools. HC-IgA with rheumatoid factor activity could also be demonstrated in the unfractionated serum pool. The binding of HC-IgA in the ELISA was not mediated through its protein HC part. The present observations show that HC-IgA carries antibody activities and constitutes a unique class of IgA complexes, since it does not dissociate under denaturating conditions after reduction. It may represent a further biological potential of the humoral immune system.
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| 4. |
- Lohmander, Stefan
(author)
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Proteoglycans of joint cartilage. Structure, function, turnover and role as markers of joint disease
- 1988
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In: Baillière's Clinical Rheumatology. - 0950-3579. ; 2:1, s. 37-62
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Journal article (peer-reviewed)abstract
- Joint cartilage consists of cells embedded in a matrix of fibrous collagen within a concentrated water-proteoglycan gel. The integrity of this matrix is crucial for the biomechanical properties of the joint cartilage. The different components of the matrix are synthesized and degraded by the cartilage cells, a process regulated by the amount of mechanical stress applied to the chondrocytes as well as by peptide factors and hormones present in synovial fluid. The proteoglycans are large macromolecules consisting of a protein core to which are attached multiple chains of glycosaminoglycans and oligosaccharides. During normal and pathological turnover, degradation products are released to the synovial fluid and to the circulation. Newly developed assays allow the sensitive and specific detection of these fragments in joint fluid and serum. Results of experimental and clinical investigations suggest that these assays will be of value in efforts to diagnose, grade and predict the outcome of inflammatory and degenerative joint disease.
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| 5. |
- Schröder, A K, et al.
(author)
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Binding of monoclonal IgM rheumatoid factor to streptococci via the antibody combining site
- 1987
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In: International Archives of Allergy and Applied Immunology. - 0020-5915. ; 83:1, s. 88-91
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Journal article (peer-reviewed)abstract
- Radiolabelled monoclonal IgM rheumatoid factors, four from patients with type II essential cryoglobulinaemia and one originating from a patient with rheumatoid arthritis, were tested for binding to group A, B, C and G streptococci and Escherichia coli. Two of the preparations exhibited different binding patterns for the streptococci, whereas the remaining three were not reactive. The binding of one of the factors to group A streptococci type 15 was inhibited by F(ab')2-fragments of anti-idiotypic antibodies but not anti-IgM Fc or F(ab')2 of normal rabbit IgG, indicating that the reaction was mediated via the antibody combining sites.
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| 6. |
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| 7. |
- Dahlqvist, Solbritt Rantapää, et al.
(author)
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Acetylator phenotypes in rheumatoid-arthritis patients with or without adverse drug-reactions to sodium-aurothiomalate or d-penicillamine
- 1987
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In: Scandinavian Journal of Rheumatology. - : Informa UK Limited. - 0300-9742 .- 1502-7732. ; 16:4, s. 235-239
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Journal article (peer-reviewed)abstract
- The acetylator phenotype was determined in 59 patients with classical, seropositive and erosive rheumatoid arthritis (RA) treated with sodium-aurothiomalate or d-penicillamine. Patients with adverse drug reactions (ADR) leading to drug withdrawal (n=29) were compared with a group of patients without ADR (n=30). The frequency of slow acetylators was significantly (p< 0.05) increased in all RA patients, irrespective of the presence of ADR, particularly in the male patients, compared with a control population. No association was found between acetylator phenotype and clinical data or secondary Sjögrens's syndrome (SS).
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| 8. |
- Dahlqvist, Solbritt Rantapää, et al.
(author)
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Clinical symptoms and HLA antigens in a family with reiters disease
- 1985
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In: Scandinavian Journal of Rheumatology. - 0300-9742 .- 1502-7732. ; 14:2, s. 149-158
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Journal article (peer-reviewed)abstract
- A clinical and immunogenetic study was performed on a three-generation family with Reiter's disease (RD). Twelve of 56 members of the family (33 clinically examined) including one in-law, had symptoms of arthritis, urethritis, conjunctivitis, uveitis, and/or mucocutaneous manifestations, but only one had the complete triad of Reiter's syndrome (RS). Radiographic sacro-iliitis was found in 7 individuals, and monoarticular onset was reported in 5 out of 7 with peripheral arthritis. HLA B27 was found in 26 of the 37 family members who were tissue typed (including one in-law). All individuals with RD were B27-positive. Seven different B27 phenotypes were identified. This finding suggests that RD is associated with the B27 antigen itself, and not to a gene closely linked to B27. From a pedigree analysis of this family an autosomal dominant inheritance with incomplete penetrance or multifactorial inheritance seemed the most probable alternatives. The family history is a useful adjunct in the diagnosis of RD.
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| 9. |
- Dahlqvist, Solbritt Rantapää
(author)
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Genetic-markers in rheumatoid-arthritis
- 1986
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In: Scandinavian Journal of Rheumatology. - 0300-9742 .- 1502-7732. ; 15:Suppl. 58, s. 1-29
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Journal article (peer-reviewed)
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| 10. |
- Dahlqvist, Solbritt Rantapää, et al.
(author)
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HLA antigens in rheumatoid-arthritis patients with and without a family history of polyarthritis
- 1985
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In: Scandinavian Journal of Rheumatology. - 0300-9742 .- 1502-7732. ; 14:4, s. 375-380
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Journal article (peer-reviewed)abstract
- The HLA antigens, A, B and DR, were studied in 141 patients with erosive, seropositive rheumatoid arthritis (RA). The frequency of the B27 antigen was significantly increased among patients with a family history of symmetrical polyarthritis compared with blood donor controls (p<0.001) and with patients without a family history of polyarthritis (p<0.005). The frequency of DR4 was significantly (p<0.001) increased among the RA patients, but there was no significant association between DR4 and a family history of polyarthritis.
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