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Träfflista för sökning "hsv:(MEDICIN OCH HÄLSOVETENSKAP) hsv:(Medicinska och farmaceutiska grundvetenskaper) hsv:(Mikrobiologi inom det medicinska området) ;lar1:(ltu)"

Search: hsv:(MEDICIN OCH HÄLSOVETENSKAP) hsv:(Medicinska och farmaceutiska grundvetenskaper) hsv:(Mikrobiologi inom det medicinska området) > Luleå University of Technology

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1.
  • Gustavsson, Martin, 1984-, et al. (author)
  • Surface Expression of omega-Transaminase in Escherichia coli
  • 2014
  • In: Applied and Environmental Microbiology. - : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 80:7, s. 2293-2298
  • Journal article (peer-reviewed)abstract
    • Chiral amines are important for the chemical and pharmaceutical industries, and there is rapidly growing interest to use transaminases for their synthesis. Since the cost of the enzyme is an important factor for process economy, the use of whole-cell biocatalysts is attractive, since expensive purification and immobilization steps can be avoided. Display of the protein on the cell surface provides a possible way to reduce the mass transfer limitations of such biocatalysts. However, transaminases need to dimerize in order to become active, and furthermore, they require the cofactor pyridoxal phosphate; consequently, successful transaminase surface expression has not been reported thus far. In this work, we produced an Arthrobacter citreus omega-transaminase in Escherichia coli using a surface display vector based on the autotransporter adhesin involved in diffuse adherence (AIDA-I), which has previously been used for display of dimeric proteins. The correct localization of the transaminase in the E. coli outer membrane and its orientation toward the cell exterior were verified. Furthermore, transaminase activity was detected exclusively in the outer membrane protein fraction, showing that successful dimerization had occurred. The transaminase was found to be present in both full-length and proteolytically degraded forms. The removal of this proteolysis is considered to be the main obstacle to achieving sufficient whole-cell transaminase activity.
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2.
  • Näslund, Annika (author)
  • Commentaries
  • 2004
  • In: International Journal of Therapy and Rehabilitation. - : MA Healthcare Ltd. - 1741-1645 .- 1759-779X. ; 11:3, s. 126-
  • Journal article (other academic/artistic)
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  • Result 1-2 of 2
Type of publication
journal article (2)
Type of content
other academic/artistic (1)
peer-reviewed (1)
Author/Editor
Larsson, Gen (1)
Gustavsson, Martin, ... (1)
Muraleedharan, Madhu ... (1)
Näslund, Annika (1)
University
Luleå University of Technology  (2)Undo refine
Royal Institute of Technology (1)
Language
English (2)
Research subject (UKÄ/SCB)
Medical and Health Sciences (2)
Engineering and Technology (1)

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