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Sökning: hsv:(NATURVETENSKAP) hsv:(Biologi) hsv:(Bioinformatik och systembiologi) > Annan publikation

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  • Carstensen, Anna-Maria, 1982, et al. (författare)
  • Method and system for growth status determination of a plant
  • 2015
  • Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
    • The present invention relates to a method fordetermining a growth status of a plant comprisingchlorophyll, the method comprising the steps of:illuminating the plant (102) with input lightincluding a light intensity modulation component(205, 206, 207, 208); detecting light emitted fromthe plant; determining (S702) an offset lightintensity (204) surrounding the plant, the offset lightintensity being a static component of the input light;determining (S718) a phase and a gain between theinput light and the detected light, determining(S720) a growth status of the plant based on apredetermined relationship between input light anddetected light, and on the phase and the gain. Theinvention also relates to a corresponding system andto a computer program product.
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  • Alneberg, Johannes, et al. (författare)
  • BARM and BalticMicrobeDB, a reference metagenome and interface to meta-omic data for the Baltic Sea
  • Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
    • The Baltic Sea is one of the world’s largest brackish water bodies and is characterised by pronounced physicochemical gradients where microbes are the main biogeochemical catalysts. Meta-omic methods provide rich information on the composition of, and activities within microbial ecosystems, but are computationally heavy to perform. We here present the BAltic Sea Reference Metagenome (BARM), complete with annotated genes to facilitate further studies with much less computational effort. The assembly is constructed using 2.6 billion metagenomic reads from 81 water samples, spanning both spatial and temporal dimensions, and contains 6.8 million genes that have been annotated for function and taxonomy. The assembly is useful as a reference, facilitating taxonomic and functional annotation of additional samples by simply mapping their reads against the assembly. This capability is demonstrated by the successful mapping and annotation of 24 external samples. In addition, we present a public web interface, BalticMicrobeDB, for interactive exploratory analysis of the dataset.
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  • Mohammed, Mubasher, Msc, et al. (författare)
  • Single-cell transcriptomics reveals transcriptional programs underlying male and female cell fate during Plasmodium falciparum gametocytogenesis
  • Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
    • The Apicomplexa constitute a large phylum of parasitic protozoa with complex life cycles that typically include meiotic sex. The life cycle of the malaria parasite, Plasmodium falciparum, includes obligate transition and stage development between a human and mosquito host. Asexual parasite replication in the human erythrocytes is followed by differentiation which leads to the formation of a precursor gamete stage, referred to as gametocytes. The gametocyte stage is solely responsible for malaria transmission into the mosquito vector where gamete fusion followed by meiosis occurs. How the parasite differentiates into male and female gametocytes in the absence of sex chromosomes largely remains an open question. Here we combine FACS-based cell enrichment of a gametocyte reporter line followed by single-cell RNA-seq, to enable targeted characterization of the entire gametocyte developmental stage. Our data defines differential transcriptional programs during male and female gametocyte development and highlights a bifurcation point for sexual cell fate. We perform prediction analyses of novel candidate driver genes underlying P. falciparum male and female lineage development. Our data indicate that a large panel of genes linked to the inner membrane complex, known to be involved in morphological life cycle changes, appears to be uniquely expressed in the female gametocyte lineage. Additionally, we delineate the timing of expression of members of the ApiAP2 family of transcription factors and predict their specificity in male and female P. falciparum gametocyte development. A motif-driven gene regulatory network analysis indicates a major role for AP2-G5 in downstream gene regulation along the male lineage developmental trajectory. In total, we anticipate that this study provides the malaria community with an important resource for the development of transmission-blocking intervention strategies.
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