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| 2. |
- Mamo, Gashaw, et al.
(författare)
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Preface
- 2020
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Ingår i: Alkaliphiles in Biotechnology. - 0724-6145. ; 172
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 3. |
- Adlercreutz, Patrick, et al.
(författare)
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Oxygen supply to immobilized cells : 1. Oxygen production by immobilized Chlorella pyrenoidosa
- 1982
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Ingår i: Enzyme and Microbial Technology. - : Elsevier BV. - 0141-0229. ; 4:5, s. 332-336
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Tidskriftsartikel (refereegranskat)abstract
- Oxygen supply at high cell densities of aerobic organisms is a great problem in immobilized cell preparations. To investigate the possibility of in situ oxygen generation the alga Chlorella pyrenoidosa was immobilized on alginate beads. The conditions for optimal oxygen production were investigated and in long term experiments the preparations were successful for at least 30 days.
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| 4. |
- Adlercreutz, Patrick, et al.
(författare)
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Oxygen supply to immobilized cells : 2. Studies on a coimmobilized algae-bacteria preparation with in situ oxygen generation
- 1982
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Ingår i: Enzyme and Microbial Technology. - : Elsevier BV. - 0141-0229. ; 4:6, s. 395-400
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Tidskriftsartikel (refereegranskat)abstract
- A coimmobilized mixed culture of algae, Chlorella pyrenoidosa, and bacteria, Gluconobacter oxydans, has been studied. The conversion of glycerol to dihydroxyacetone(1,3-dihydroxy-2-propanone), catalysed by the bacteria, was used to indicate the oxygen supply in the immobilized preparation. The oxygen produced by the algae in the coimmobilized preparation was used by the bacteria more effectively than when the cells were immobilized separately and mixed within the reactor. A preparation consisting of only bacteria and no algae was much less effective. The coimmobilized preparation was used in the continuous production of dihydroxyacetone for six days without any significant loss of activity.
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| 5. |
- Adlercreutz, Patrick, et al.
(författare)
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Oxygen supply to immobilized cells - 3. Oxygen supply by hemoglobin or emulsions of perfluorochemicals
- 1982
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Ingår i: European Journal of Applied Microbiology and Biotechnology. - 0171-1741. ; 16:4, s. 165-170
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Tidskriftsartikel (refereegranskat)abstract
- Oxygen supply is a critical point in technical processes when aerobic cells are used in immobilized preparations. This report concerns the use of hemoglobin or emulsions of perfluorochemicals (completely fluorinated organic compounds) to carry oxygen to immobilized cells. Both methods work well and do not seem to harm the cells. The perfluorochemical method of improving oxygen supply showed a higher operational stability than the hemoglobin method.
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| 8. |
- Ahlqvist, Josefin, et al.
(författare)
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Monitoring the production of inclusion bodies during fermentation and enzyme-linked immunosorbent assay analysis of intact inclusion bodies using cryogel minicolumn plates
- 2006
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Ingår i: Analytical Biochemistry. - : Elsevier BV. - 1096-0309 .- 0003-2697. ; 354:2, s. 229-237
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Tidskriftsartikel (refereegranskat)abstract
- A novel minicolumn chromatgraphic method to monitor the production of inclusion bodies during fermentation and anenzyme-linked immunosorbent assay (ELISA) system allowing direct analysis of the particles with surface-displayed antigens are described. A 33-kDa protein containing 306 amino acids with three sulfur bridges produced its inclusion bodies wits labeled with polyclonal antibodies against 15 amino acid (anti-A15) and 17 amino acid (anti-B17) residues at the N- and C-terminal ends of the protein, respectively. Labeled particles were bound to macroporous Monolithic protein A-cryogel adsorbents inserted into the open-ended wells of a 96-well plate (referred to as protein A-cryogel minicolumn plate). The concept behind this application is that the binding degree of inclusion bodies from lysed fermentation broth to the cryogel minicolumns increases with an increase in their concentration during fermentation. The technique allowed LIS to monitor the increase in the production levels of the inclusion bodies as the fermentation process progressed. The system also has a built-in quality parameter to ensure that the target protein has been fully expressed. Alternatively, inclusion bodies immobilized on phenyl-cryogel minicolumn plate were used in indirect ELISA based on anti-A15 and anti-B17 antibodies against terminal amino acid residues displayed oil the surface of inclusion bodies. Drainage-protected properties of the cryogel minicolumns allow performance of successive reactions with tested immunoglobulin G (IgG) samples and enzyme-conjugated secondary I-G and of enzymatic reaction within the adsorbent. (c) 2006 Elsevier Inc. All rights reserved.
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| 9. |
- Alvarez, Maria Teresa, et al.
(författare)
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Lab-scale production of biogenic sulphide for metal precipitation in remote areas
- 2012
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Ingår i: International Journal of Environment and Waste Management. - 1478-9876. ; 9:3-4, s. 313-329
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Tidskriftsartikel (refereegranskat)abstract
- Batch cultures with wheat straw, biomass of Paja Brava (Festuca orthophylla), filter paper, newspaper and beech leaves (Fagus sylvatica) were established to produce sulphide. Sulphide production, sulphate reduction, concentration of Volatile Fatty Acids (VFAs), enzyme activities and Fluorescence in situ hybridisation were determined. Approximately 5 mM of sulphide was produced during anaerobic digestion of wheat straw, while the production with newspaper as carbon source was the lowest (ca.1 mM). The sulphide production (2-5 mM) in the semi-continuous culture of the consortium A10, using wheat straw supported Cu(II), Pb (II) and Zn (II) removal up to 90%.
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| 10. |
- Andac, M, et al.
(författare)
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Poly(hydroxyethyl methacrylate)-based macroporous hydrogels with disulfide cross-linker
- 2008
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Ingår i: Macromolecular Chemistry and Physics. - : Wiley. - 1521-3935 .- 1022-1352. ; 209:6, s. 577-584
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Tidskriftsartikel (refereegranskat)abstract
- Biodegradable supermacroporous PHEMA cryogels were produced by combining two cross-linkers, poly(ethylene glycol) diacrylate and a newly developed disulfide water soluble crosslinker, N,N'-bis(methacryloyl)-L-cystine. The biodegradable PHEMA cryogels were prepared with gel fraction yields up to 70% and were characterized by highly interconnected pores of micrometer size and good mechanical stability. When subjected to reductive agents like DTT, the biodegradable PHEMA cryogels disintegrated into small pieces. The rate of disintegration was controlled by the crosslinking density in the cryogels and the DTT concentration.
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