| 1. |
- Chester, Alan, et al.
(författare)
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Biosynthesis of the blood-group-B-specific trisaccharide in a rhesus monkey
- 1977
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Ingår i: European Journal of Biochemistry. - 0014-2956. ; 77:1, s. 87-91
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Tidskriftsartikel (refereegranskat)abstract
- A Rhesus monkey, serologically grouped as B, has been shown to excrete low-molecular-weight carbohydrate material in urine closely related to that found in human urine. Galactose feeding resulted in the excretion of a trisaccharide which was shown to be identical to the trisaccharide isolated from the urine of group B humans under the same conditions. Experiments in which [14C]galactose was administered both orally and via an intestinal vein demonstrated that the intestine is the site of biosynthesis.
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| 2. |
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| 3. |
- Chester, Alan, et al.
(författare)
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Phenyl beta-D-Galactopyranoside as an Acceptor Substrate for the Blood-Group H Gene-Associated Guanosine Diphosphate L-Fucose:beta-D-Galactosyl alpha-2-L-Fucosyltransferase
- 1976
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Ingår i: European Journal of Biochemistry. - 0014-2956. ; 69:2, s. 583-592
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Tidskriftsartikel (refereegranskat)abstract
- Phenyl β-d-galactopyranoside was found to be an efficient acceptor of l-[14C]fucose when guanosine diphosphate l-[14C]fucose was used as the donor substrate and human serum, submaxillary glands or stomach mucosa were the sources of l-fucosyltransferase. The enzyme utilising phenyl β-D-galactoside was present in the serum of donors of all ABO blood-groups examined, except those of the rare Oh (Bombay) and Bh phenotypes, but was clearly demonstrable in submaxillary gland preparations only when the glands came from individuals who were secretors of ABH blood-group substances. This distribution coincides with that previously established for the blood-group H gene-specified α-2-l-fucosyltransferase. The product of l-[14C]fucosyl transfer to phenyl β-d-galactoside is separable from the other radioactive components in the enzyme digest by chromatography for 4 h in ethyl acetate/pyridine/water (10/4/3, by vol.); it was characterised as phenyl 2-O-(α-l-[14C]fucopyranosyl)β-d-galactopyranoside by (a) the liberation of l-[14C]fucose by a specific α-2-l-fucosidase, (b) its resistance to degradation by alkali and (c) the identification of tritiated glycerol as a product of periodate oxidation after a 3H label had been introduced into the galactosyl moiety. The use of phenyl β-d-galactopyranoside as acceptor substrate thus provides a simple and relatively rapid method for the assay of the blood-group H gene-specified α-2-l-fucosyltransferase.
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| 4. |
- Chester, Alan, et al.
(författare)
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Urinary excretion of oligosaccharides induced by galactose given orally or intravenously
- 1979
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Ingår i: European Journal of Biochemistry. - 0014-2956. ; 100:2, s. 385-392
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Tidskriftsartikel (refereegranskat)abstract
- The effect of oral administration of galactose, lactose, and sucrose and intravenous injection of galactose on the urinary excretion of blood-group-active oligosaccharides has been studied. Galactose given either as the free sugar, a glycoside (lactose) or a constituent of normal diet was an absolute requirement for the formation and excretion of A-trisaccharide, B-trisaccharide and 2'-fucosylgalactose in blood group A, B and O(H) secretors, respectively. Great individual variation was seen in the amounts of galactose-dependent oligosaccharides excreted. Injection of galactose resulted in excretion of 3-59% of the amount of oligosaccharide formed after oral administration to the same individual. The mean ratio A-trisaccharide/B-trisaccharide was 2.7 in four blood-group-A1B secretors and 0.22 in three A2B secretors and can thus serve as a parameter for chemical differentiation between the two blood groups. The excretion of larger blood-group-active oligosaccharides, including the A-pentasaccharide, the B-pentasaccharide and lactodifucotetraose, that are normal components in urine from, respectively, starved A, B, and H secretors, was about the same after oral administration of galactose or lactose. The B-trisaccharide was the only oligosaccharide detected in plasma after oral galactose administration to a blood-group-B secretor individual. The concentration was 0.38 mg/l of plasma.
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| 5. |
- Holmberg, Lars, et al.
(författare)
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Measurement of antihaemophilic factor A antigen (VII:CAg) with a solid phase immunoradiometric method based on homologous non-haemophilic antibodies.
- 1979
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Ingår i: Scandinavian Journal of Haematology. - 0036-553X. ; 23:1, s. 17-24
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Tidskriftsartikel (refereegranskat)abstract
- Antihaemophilic-factor-A-antibodies, which had spontaneously arisen in 2 patients, were used to develop an immunoradiometric method for measurement of antihaemophilic factor A antigen (VIII:CAg). 13 patients with severe haemophilia A had VIII: CAg below the limit of detection (0.01 U/ml). Patients with moderate and mild haemophilia A either had VIII:CAg roughly equal to factor VIII clotting activity (VIII:C) or a not detectable VIII:CAg, suggesting 2 different molecular mechanisms in moderate and mild haemophilia A. VIII:CAg could be detected in serum but in lower amounts than in plasma. In 2 patients with von Willebrand's disease VIII:CAg equalled VIII:C. The post-transfusional retarded increase of VIII:C in 1 patient with von Willebrand's disease was accompanied by a slight increase in VIII:CAg. Fetal plasma contained measurable amounts of VIII:CAg.
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| 6. |
- Ljung, R., et al.
(författare)
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Purification of F.VIII:C by antigen-antibody chromatography
- 1978
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Ingår i: Thrombosis Research. - : Elsevier BV. - 0049-3848. ; 12:4, s. 667-675
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Tidskriftsartikel (refereegranskat)abstract
- Purification of F.VIII:C devoid of F.VIII:Ag was achieved by antigen-antibody chromatography. The antibody used neutralized VIIIR:Ag but not VIII:C in liquid phase but extracted both VIII:Ag and VIII:C from plasma when bound to Sepharose. VIII:C was eluted with calcium-containing buffer. When plasma was used as starting material VIII:C was obtained free from VIIIR:Ag but contaminated with some other proteins. When a well-defined pure F.VIII preparation was used as starting material the VIII:C active fractions contained no immunoradiometrically detectable VIIIR:Ag, no VIIIR:RCF and no detectable protein. When stabilized with albumin VIII:C could be frozen and thawed with retained activity and could be activated with thrombin.
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| 7. |
- Renlund, Martin, et al.
(författare)
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Increased urinary excretion of free N-acetylneuraminic acid in thirteen patients with Salla disease
- 1979
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Ingår i: European Journal of Biochemistry. - 0014-2956. ; 101:1, s. 245-250
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Tidskriftsartikel (refereegranskat)abstract
- Thirteen severely retarded patients with Salla disease, a new type of lysosomal storage disorder, have been studied biochemically. All patients excreted approximately ten times more free sialic acid than normal individuals. The isolated sialic acid was characterized by paper chromatography, thin-layer chromatography, optical rotation, 13C and 1H nuclear magnetic resonance spectroscopy, and mass spectrometry of its permethylated derivative. The results clearly indicated that the excreted sialic acid was identical to N-acetylneuraminic acid. The main sialylated trisaccharide present in the urine of the patients was identified as 3'-sialyllactose by sugar and methylation analysis. The excreted amounts were found to be within normal range.
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| 8. |
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