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- Belfrage, Per, et al.
(författare)
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Dispersion of viable pig liver cells with collagenase
- 1975
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Ingår i: Life Sciences. - : Elsevier BV. - 1879-0631 .- 0024-3205. ; 17:8, s. 1219-1225
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Tidskriftsartikel (refereegranskat)abstract
- Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.
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| 3. |
- Hultborn, Ragnar, 1946, et al.
(författare)
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Prolonged activation of soleus motoneurones following a conditioning train in soleus Ia afferents - A case for a reverberating loop?
- 1975
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Ingår i: Neuroscience letters. - 0304-3940. ; 1:3, s. 147-52
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Tidskriftsartikel (refereegranskat)abstract
- In the decerebrate cat a short train of impulses in Ia afferents from the soleus muscle (or its synergists) may cause a long latency prolonged activity in the soleus muscle as judged by EMG and tension recording. The excitability increase may stay virtually constant during long periods (several minutes) but can be terminated at any time by a train of impulses in, for example, the peroneal nerve. It is suggested that the conditioning Ia impulses activate a neuronal circuit which can maintain a reverberating activity thereby causing the heightened excitability of the soleus motoneurones.
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| 9. |
- Janunger, Karl-Gunnar, 1941-
(författare)
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Benign and malignant gastric mucosal changes after partial gastrectomy
- 1978
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The development of benign and malignant mucosal changes in the gastric remnant were studied early (1-3 years) postoperatively in 55 patients and late (10-24 years) postoperatively in 336 of 676 patients subjected to partial gastrectomy for ulcer disease.Chronic gastritis with atrophy, intestinal metaplasia and cystic dilatation of the gastric glands was found early postoperatively with the same prevalence in gastric and duodenal ulcer patients. Whereas the prevalence of atrophy, and of acute and chronic gastritis was the same both early and late postoperatively, the prevalence of intestinal metaplasia, cystic dilatation and lipid islands increased with time. The prevalence of inflammatory changes late postoperatively was not correlated to sex, age or type of anastomotic procedure.A significantly increased risk for stump carcinoma was found in male patients more than 12 years postoperatively. No difference correlated to type of ulcer disease or anastomotic procedure could be demonstrated. In duodenal ulcer patients the time interval between operation and diagnosis of carcinoma was independent of age at operation, while in gastric ulcer patients the interval was shorter with increasing age at operation.Gastric stump carcinomas were found in 12 of the 336 (3.6%) patients examined gastroscopically; four of these were early carcinomas. In four patients with stump carcinoma the correct diagnosis could not be established at the first examination.Gastric polyps and precancerous mucosal changes were the only macro- or microscopical findings in some cases with already existing non-visible carcinoma.The CEA immunohistochemical study of the gastric mucosa showed a positive reaction in 10 of 49 patients; 6 of the 10 had carcinoma, precancerous changes or adenomatous polyp. Three patients with diagnosed or later discovered carcinoma had CEA negative reactions. However, the results indicate that there is a correlation between demonstrated CEA content and increased risk for development of stump carcinoma. To evaluate whether this method can be used to identify patients at special risk for development of gastric carcinoma requires further study.Because of increased risk for stump carcinoma, gastric ulcer patients ought to be examined with gastroscopy from about 10 years after partial gastrectomy, and duodenal ulcer patients from about 15 years, irrespective of the type of anastomotic procedure. Re-examinations ought to be performed every two to four years. In patients with gastric polyps or precancerous mucosal changes re-examinations should be performed earlier, within 6-12 months.
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| 10. |
- Malmström, Per, et al.
(författare)
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Cholecystomy for acute choelcystitis
- 1973
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Ingår i: The American Journal of Surgery. - 1879-1883. ; 126:3, s. 397-402
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Tidskriftsartikel (refereegranskat)abstract
- During the decade 1957 through 1966, sixty-three cholecystostomies in sixty-two patients were performed in the Surgical Department, University of Lund. Fifty-five of these sixty-two patients were followed up. The mean age was seventy-one years for both sexes. The primary mortality was 20 per cent: a third died of circulatory and respiratory insufficiency; three quarters died of peritonitis, abscess, and renal failure; half of the surviving patients had delayed wound healing. The mean hospital stay for the survivors was nineteen days. During the follow-up period, sixteen later required cholecystectomy, nine electively and seven for a recurrence of acute cholecystitis. Nine had another attack of cholecystitis after cholecystostomy. Another four had continuous symptoms; five more died of the gallbladder disease.It is concluded that cholecystostomy should be restricted to very ill patients and should be planned and carried out with the patient under local anesthesia. In all other patients, early primary cholecystectomy should be performed with cholangiography performed during the operation.
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