| 1. |
- Børresen, A L, et al.
(författare)
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Prenatal diagnosis of glycerol-kinase deficiency associated with a DNA deletion on the short arm of the X-chromosome.
- 1987
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Ingår i: Clinical genetics. - 0009-9163. ; 32:4, s. 254-9
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Tidskriftsartikel (refereegranskat)abstract
- Amniocentesis was performed in a woman who previously had given birth to a boy who died at 12 months of age with a diagnosis of glyceroluria and adrenal insufficiency. A high amount of glycerol (9.0 standard deviations above mean for controls) was found in the amniotic fluid. Enzyme activity of glycerol-kinase (ATP:glycerol-3-phosphotransferase, EC 2.7.1.30) in the cultured amniotic fluid cells was very low. The pregnancy was terminated and a male fetus was aborted. Examinations of DNA isolated from the fetus did demonstrate deletions of two out of 16 DNA probes mapping to the short arm of the X-chromosome. The probes failing to hybridize to DNA from the fetus were C7 (DXS28) and L1.4 (DXS68), both mapping to Xp21.3 and located terminal to the Duchenne locus.
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| 2. |
- Sjöberg, Lennart, et al.
(författare)
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A comparison between Bactec aerobic resin and hypertonic blood culture media.
- 1988
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Ingår i: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS). - : Wiley. - 0903-4641 .- 1600-0463. ; 96:8, s. 720-722
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Tidskriftsartikel (refereegranskat)abstract
- The presence of antimicrobial agents in patients' blood is thought to represent an important source of false-negative blood cultures. This has led to the incorporation of agents with inhibitory effects on antimicrobial drugs into culture medium. In the present study, Bactec aerobic resin-containing blood culture medium was compared with Bactec hypertonic blood culture medium. 504 patients receiving cytostatic and/or antibiotic treatment were studied. Sensitivity calculations on detection of bacteremia in these patients gave 0.91 for the resin medium and 0.79 for the hypertonic blood culture system and showed a significant difference (p = 0.016). In addition, the resin-containing system more rapidly detected positive cultures than the hypertonic system.
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| 3. |
- Wirth, Michael, 1956, et al.
(författare)
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Interactions between DNA and mono-, bis-, tris-, tetrakis-, and hexakis(aminoacridines). A linear and circular dichroism, electric orientation relaxation, viscometry, and equilibrium study
- 1988
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 110:3, s. 932-939
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Tidskriftsartikel (refereegranskat)abstract
- The interaction between DNA and a series of mono-, bis-, tris-, tetrakis-, and hexakis-intercalating 9-aminoacridines has been studied with flow linear dichroism (LD), circular dichroism (CD), electric orientation relaxation (EOR) techniques, and with viscometry and equilibrium analyses. The orientation of the 9-aminoacridine ligand relative to the average orientation of the DNA bases, measured by LD, shows that with both 9-aminoacridine and the bis(acridines) the in-plane short axes of the acridine ligands are oriented perfectly parallel to the planes of the DNA bases, as expected for classical intercalation, whereas the long axes are found to be significantly tilted. This is supported by the DNA lengthening measured by EOR, which for 9-aminoacridine is 1.5 base-pair units, compared with 1.0 for ethidium bromide. Also in case of the tris(acridines) LD, CD, viscometry, and equilibrium data indicate that all acridine ligands are intercalated. The binding analysis shows an increasing degree of cooperativity in the sequence 9-aminoacridine < bis(acridines) < tris(acridines), and the corresponding binding densities, 4, 8, and 11–14, respectively, are in good agreement with those expected from the nearest-neighbor exclusion principle. The LD and CD measurements show that the tetrakis- and hexakis(acridines), despite long and flexible links, bind to DNA with only three of the acridine ligands intercalated.
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| 4. |
- Nilsonne, Åsa, et al.
(författare)
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Measuring the rate of change of voice fundamental frequency in fluent speech during mental depression
- 1988
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Ingår i: The Journal of the Acoustical Society of America. - : American Institute of Physics (AIP). - 0001-4966. ; 83:2, s. 716-728
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Tidskriftsartikel (refereegranskat)abstract
- A method of measuring the rate of change of fundamental frequency has been developed in an effort to find acoustic voice parameters that could be useful in psychiatric research. A minicomputer program was used to extract seven parameters from the fundamental frequency contour of tape‐recorded speech samples: (1) the average rate of change of the fundamental frequency and (2) its standard deviation, (3) the absolute rate of fundamental frequency change, (4) the total reading time, (5) the percent pause time of the total reading time, (6) the mean, and (7) the standard deviation of the fundamental frequency distribution. The method is demonstrated on (a) a material consisting of synthetic speech and (b) voice recordings of depressed patients who were examined during depression and after improvement.
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| 5. |
- Gustafsson, Hans, 1952-
(författare)
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Salivary gland neoplasms : studies on the cytoskeleton, the secretory apparatus and the nuclear DNA content
- 1986
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The heterogeneity of salivary gland neoplasms have made classification and prognostication of these tumours sometimes difficult, and the introduction of techniques, such as enzyme and carbohydrate histochemistry and electron microscopy have only to a certain extent increased our knowledge in these respects. In the present study immunohistochemical methods have been used to identify intermediate filament proteins (IFP) in normal fetal and adult parotid glands, as well as in salivary neoplasms. The intermediate filaments (IF) make up the cytoskeleton in eucaryotic cells. Epithelial tissue contains IF composed of different cytokeratins (CK 1-19) whilst mesenchymal tissue generally contains IF composed of vimentin, and the IFP pattern is very stable even during cell transformation. It would thus be possible to further clarify the histogenesis of salivary neoplasms by identifying IFP, in addition the IFP pattern would probably be useful in tumour typing. Furthermore, ultrastructural cytochemical studies, microspectorphotometry on nuclear DNA as well as enzyme secretory studies of certain tumour types were carried out, in order to further characterize the biology of salivary neoplasms.The immunohistochemical investigations showed that in normal parotid tissue, the different cell types differed in IFP expression: acinar cells express mainly CK 18 and myoepithelial cells mainly CK 17 and 19, whilst duct cells contained a broad range of CK. Vimentin could in addition to CK be detected in myoepithelial cells and basal cells of excretory ducts. Fetal parotid cells showed a similar CK pattern as mature duct cells. In addition, vimentin could be found in some basal cells of the terminal tubules of the fetal glands. Salivary neoplasms could be divided into three types with regard to their IFP pattern: Acinic cell carcinomas showed a CK-pattern similar to normal acinar cells but a co-expression of CK and vimentin was present in some cells. Adenoid cystic carcinomas, mixed tumours and basal cell adenomas showed a CK-pattern of normal duct or myoepithelial cells. The peripheral cells were also vimentin positive. 3. Mucoepidermoid carcinomas and adenocarcinomas had a similar CK-pattern as duct cells, and no tumour cells contained vimentin. This indicates that typing of IFP may be useful for subgrouping of salivary neoplasms.By stereological measurements, the cells of acinic cell carcinomas were found to be very similar to normal parotid acinar cells. Furthermore, they contained amylase and after stimulation by norepiphrine a secretory response was induced, with a rise in intracellular cAMP as well as a release of amylase. By single cell measurements of nuclear DNA content, no difference was found between acinic cell carcinomas with definite metastasis and those without recurrence, both in paraffin sections and cytological smears.
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| 6. |
- Biberfeld, G, et al.
(författare)
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Enzyme immunoassays for the demonstration of antibodies to HIV-2SBL-6669 and HTLV-IV (SIVmac).
- 1988
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Ingår i: AIDS. - 0269-9370 .- 1473-5571. ; 2:3, s. 195-9
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Tidskriftsartikel (refereegranskat)abstract
- Enzyme-linked immunosorbent assays (ELISA) were developed for the demonstration of antibodies to HIV-2 using disrupted virions of the SBL-6669 isolate of HIV-2 and the so-called human T-lymphotropic virus type IV (HTLV-IV), recently found to be identical with the simian immunodeficiency virus (SIVmac), as antigens. Three hundred sera from West African subjects, attending an outward clinic in Bissau for examination of suspected tuberculosis, were tested by these two assays as well as by a commercially available anti-HIV-2 ELISA (ELAVIA II). Fifty of these sera were positive in all three ELISAs as well as in Western blot tests against HTLV-IV. Thirty-eight of these positive sera were also tested by an anti-HIV-2 Western blot kit (LAV-Blot II) with positive results. The ELISAs based on SBL-6669 and HTLV-IV antigens had a specificity of 99.6% (one false positive among 250 negative sera) whereas the specificity of ELAVIA II was 94.6% using the recommended cut-off value and 98.4% using a higher cut-off value. Another 58 sera from West African patients, clinically suspected of having AIDS or HIV-related disease, were tested for HIV-2/HTLV-IV antibodies by Western blot and by ELISA against SBL-6669 and HTLV-IV antigens; all of the 30 sera which were positive by Western blot were found to be positive in both ELISAs.(ABSTRACT TRUNCATED AT 250 WORDS)
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| 7. |
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| 8. |
- Teriö, H, et al.
(författare)
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Estimation of elastic properties in the urethral flow controlling zone by signal analysis of urodynamic pressure/flow data.
- 1989
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Ingår i: Medical and Biological Engineering and Computing. - 0140-0118 .- 1741-0444. ; 27:3, s. 314-321
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Tidskriftsartikel (refereegranskat)abstract
- When urethral flow is treated as a lossless flow through an elastic tube, the relationship between the detrusor pressure and the urinary flow can be related to the elasticity of the flow-controlling zone of the urethra. A recent analytical method of describing urethral elasticity is implemented on a computer. The function p(Q) = pmo + LmQm is fitted to the recorded pressure/flow data. p(Q) is the detrusor pressure, Q the flow and pmo, m and Lm parameters. The elastic properties are then obtained as p(A) = pmo + KnAn, where p(A) is the static pressure, A the cross-sectional area of the flow-controlling zone and n and Kn calculated parameters. The urodynamic methods used and the computer implementation of the analytical method are described. In obstructed and unobstructed men without neurological symptoms, the elastic properties could be estimated in 94 per cent of the micturitions. The method makes it possible to describe urethral flow properties with Griffiths' model in a standardised way and compare results obtained by different investigators. It is recommended for quantification of urethral obstruction in research and for assessment of borderline cases of obstruction in clinical practice.
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