Sökning: id:"swepub:oai:DiVA.org:kth-296145" >
Stress-induced tran...
Stress-induced transcriptional memory accelerates promoter-proximal pause release and decelerates termination over mitotic divisions
-
- Vihervaara, Anniina (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,Abo Akad Univ, Fac Sci & Engn, Cell Biol, Turku 20520, Finland.;Univ Turku, Turku Biosci Ctr, Turku 20520, Finland.;Abo Akad Univ, Turku 20520, Finland.;Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA.
-
- Mahat, Dig Bijay (författare)
- Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA.;MIT, 77 Massachusetts Ave, Cambridge, MA 02139 USA.
-
- Himanen, Samu, V (författare)
- Abo Akad Univ, Fac Sci & Engn, Cell Biol, Turku 20520, Finland.;Univ Turku, Turku Biosci Ctr, Turku 20520, Finland.;Abo Akad Univ, Turku 20520, Finland.
-
visa fler...
-
- Blom, Malin A. H. (författare)
- Abo Akad Univ, Fac Sci & Engn, Cell Biol, Turku 20520, Finland.;Univ Turku, Turku Biosci Ctr, Turku 20520, Finland.;Abo Akad Univ, Turku 20520, Finland.
-
- Lis, John T. (författare)
- Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA.
-
- Sistonen, Lea (författare)
- Abo Akad Univ, Fac Sci & Engn, Cell Biol, Turku 20520, Finland.;Univ Turku, Turku Biosci Ctr, Turku 20520, Finland.;Abo Akad Univ, Turku 20520, Finland.
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- Engelska.
-
Ingår i: Molecular Cell. - : Elsevier BV. - 1097-2765 .- 1097-4164. ; 81:8, s. 1715-
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Heat shock instantly reprograms transcription. Whether gene and enhancer transcription fully recover from stress and whether stress establishes a memory by provoking transcription regulation that persists through mitosis remained unknown. Here, we measured nascent transcription and chromatin accessibility in unconditioned cells and in the daughters of stress-exposed cells. Tracking transcription genome-wide at nucleotide-resolution revealed that cells precisely restored RNA polymerase II (Pol II) distribution at gene bodies and enhancers upon recovery from stress. However, a single heat exposure in embryonic fibroblasts primed a faster gene induction in their daughter cells by increasing promoter-proximal Pol II pausing and by accelerating the pause release. In K562 erythroleukemia cells, repeated stress refined basal and heat-induced transcription over mitotic division and decelerated termination-coupled pre-mRNA processing. The slower termination retained transcripts on the chromatin and reduced recycling of Pol II. These results demonstrate that heat-induced transcriptional memory acts through promoter-proximal pause release and pre-mRNA processing at transcription termination.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas