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Laboratory phase-co...
Laboratory phase-contrast nanotomography of unstained Bombus terrestris compound eyes
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- Romell, Jenny (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Jie, Vun Wen (författare)
- Stockholms universitet,Zoologiska institutionen
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Miettinen, A. (författare)
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- Baird, Emily (författare)
- Stockholms universitet,Zoologiska institutionen
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- Hertz, Hans (författare)
- KTH,Fysik,Biomedicinsk fysik och röntgenfysik
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(creator_code:org_t)
- 2021-05-08
- 2021
- Engelska.
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Ingår i: Journal of Microscopy. - : Wiley. - 0022-2720 .- 1365-2818. ; 283:1, s. 29-40
- Relaterad länk:
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https://doi.org/10.1...
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Imaging the visual systems of bumblebees and other pollinating insects may increase understanding of their dependence on specific habitats and how they will be affected by climate change. Current high-resolution imaging methods are either limited to two dimensions (light- and electron microscopy) or have limited access (synchrotron radiation x-ray tomography). For x-ray imaging, heavy metal stains are often used to increase contrast. Here, we present micron-resolution imaging of compound eyes of buff-tailed bumblebees (Bombus terrestris) using a table-top x-ray nanotomography (nano-CT) system. By propagation-based phase-contrast imaging, the use of stains was avoided and the microanatomy could more accurately be reconstructed than in samples stained with phosphotungstic acid or osmium tetroxide. The findings in the nano-CT images of the compound eye were confirmed by comparisons with light- and transmission electron microscopy of the same sample and finally, comparisons to synchrotron radiation tomography as well as to a commercial micro-CT system were done.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
Nyckelord
- bumblebee
- compound eyes
- laboratory nano-CT
- phase contrast
- system comparison
- glucose
- glutaraldehyde
- nanotube
- osmium tetraoxide
- paraformaldehyde
- phosphotungstic acid
- acceleration
- Article
- basement membrane
- Bombus terrestris
- comparative study
- compound eye
- controlled study
- electric field
- electron microscopy
- histology
- illumination
- image processing
- micro-computed tomography
- nonhuman
- phase contrast microscopy
- photoreceptor cell
- reconstruction algorithm
- signal noise ratio
- transmission electron microscopy
- animal
- bee
- devices
- laboratory
- procedures
- synchrotron
- x-ray computed tomography
- Animals
- Bees
- Laboratories
- Microscopy
- Phase-Contrast
- Osmium Tetroxide
- Synchrotrons
- Tomography
- X-Ray Computed
- X-Ray Microtomography
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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