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Molecular and catal...
Molecular and catalytic characterization of a phi class glutathione transferase from Cathaya argyrophylla
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Wei, Tao (författare)
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Wang, Cai-Ling (författare)
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Kao, Hong-Na (författare)
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Yang, Hai-Ling (författare)
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- Wang, Xiao-Ru (författare)
- Umeå universitet,Institutionen för ekologi, miljö och geovetenskap
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Zeng, Qing-Yin (författare)
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(creator_code:org_t)
- Oxford : Pergamon Press, 2012
- 2012
- Engelska.
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Ingår i: Biochemical Systematics and Ecology. - Oxford : Pergamon Press. - 0305-1978 .- 1873-2925. ; 40, s. 75-85
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Plant phi class glutathione transferases (GSTs) play important roles in stress tolerance and detoxification metabolism. This study reports the cloning, expression and biochemical characteristics of a phi GST gene (CaGSTF) from the endemic and endangered conifer Cathaya argyrophylla. The recombinant CaGSTF showed GSH-conjugating activity towards the substrate NED-Cl and CDNB. Kinetic analysis revealed low catalytic efficiency with a k(cat)/K-m(GSH) value of 9.82 mM(-1)S(-1). The CaGSTF proved to be a thermolabile enzyme, at 40 degrees C the enzyme's activity was nearly abolished. Site-directed mutagenesis revealed that Ser12, Lys42, Ile55, Glu67 and Ser68 of CaGSTF are critical components of glutathione-binding sites that contribute to the enzyme's catalytic activity. Compared to other plant phi GSTs and conifer tau GSTs, CaGSTF showed a narrow substrate spectrum, low catalytic efficiency and thermolability. These atypical properties suggest the enzyme may have a limited functional role in the organism's adaptation to environmental stresses in the subtropical regions. (C) 2011 Elsevier Ltd. All rights reserved.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Cathaya argyrophylla
- Gymnosperm
- Enzyme characterization
- Site-directed mutagenesis
- Protein structure
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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