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Preparation of Prot...
Preparation of Protein-Enriched Outer Membrane Vesicles from Escherichia Coli for In Situ Structural Biology of Outer Membrane Proteins
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- Castegnaro, Filippo, 1994 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Wallenberg Centre for Molecular and Translational Medicine,Department of Chemistry and Molecular Biology
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- Burmann, Björn Marcus, 1979 (författare)
- Gothenburg University,Göteborgs universitet,Wallenberg Centre for Molecular and Translational Medicine,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Thoma, Johannes, 1985 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Wallenberg Centre for Molecular and Translational Medicine,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- 2023
- 2023
- Engelska.
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Ingår i: Methods in molecular biology (Clifton, N.J.). - 1940-6029. ; , s. 247-257
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Bacterial outer membrane vesicles (OMVs) can be selectively enriched with one or more outer membrane proteins to allow the biophysical characterization of these membrane proteins embedded in the native cellular environment. Unlike reconstituted artificial membrane environments, OMVs maintain the native lipid composition as well as the lipid asymmetry of bacterial outer membranes. Here, we describe in detail the steps necessary to prepare OMVs, which contain high levels of a designated protein of interest, and which are of sufficient homogeneity and purity to perform biophysical characterizations using high-resolution methods such as atomic force microscopy, electron microscopy, or single-molecule force spectroscopy.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Nyckelord
- Gram-negative bacteria
- In-situ protein studies
- Outer membrane proteins
- Outer membrane vesicles
- Protein biophysics
Publikations- och innehållstyp
- ref (ämneskategori)
- kap (ämneskategori)
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