Tyck till om SwePub Sök
här!
Search: onr:"swepub:oai:DiVA.org:umu-117443"
> Wang Xian liang >
Quantification for ...
Quantification for total demethylation potential of environmental samples utilizing the EGFP reporter gene
-
Qian, Yan (author)
-
Wang, Xiao-li (author)
-
Lv, Zhan-lu (author)
-
show more...
-
- Tysklind, Mats (author)
- Umeå universitet,Kemiska institutionen
-
Guo, Chen (author)
-
Liang, Bao (author)
-
Wu, Jia-bing (author)
-
Yang, Yong-jian (author)
-
Yang, Yi-shu (author)
-
Wang, Fei-fei (author)
-
Duan, Xiao-li (author)
-
Ma, Jin (author)
-
Wei, Yong-jie (author)
-
Wang, Chun-hui (author)
-
Yang, Li-xin (author)
-
Zhang, Jin-liang (author)
-
Shi, Xiao-ming (author)
-
Wang, Xian-liang (author)
-
show less...
-
(creator_code:org_t)
- Elsevier, 2016
- 2016
- English.
-
In: Journal of Hazardous Materials. - : Elsevier. - 0304-3894 .- 1873-3336. ; 306, s. 278-285
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Abstract The demethylation potential of pollutants is arguably an innate component of their toxicity in environmental samples. A method was developed for determining the total demethylation potential of food samples (TDQ). The demethylation epigenetic toxicity was determined using the Hep G2 cell line transfected with pEGFP-C3 plasmids containing a methylated promoter of the EGFP reporter gene. The total demethylation potential of the sample extracts (the 5-AZA-CdR demethylation toxic equivalency) can be quantified within one week by using a standard curve of the 5-AZA-CdR demethylation agent. To explore the applicability of TDQ for environmental samples, 17 groundwater samples were collected from heavy polluted Kuihe river and the total demethylation potentials of the sample extracts were measured successfully. Meaningful demethylation toxic equivalencies ranging from 0.00050 to 0.01747 μM were found in all groundwater sample extracts. Among 19 kinds of inorganic substance, As and Cd played important roles for individual contribution to the total demethylation epigenetic toxicity. The TDQ assay is reliable and fast for quantifying the DNA demethylation potential of environmental sample extracts, which may improve epigenetic toxicity evaluations for human risk assessment, and the consistent consuming of groundwater alongside the Kuihe river pose unexpected epigenetic health risk to the local residents.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Demethylation potential
- Environmental sample
- Kuihe river
- Epigenetic toxicity
- EGFP
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Qian, Yan
-
Wang, Xiao-li
-
Lv, Zhan-lu
-
Tysklind, Mats
-
Guo, Chen
-
Liang, Bao
-
show more...
-
Wu, Jia-bing
-
Yang, Yong-jian
-
Yang, Yi-shu
-
Wang, Fei-fei
-
Duan, Xiao-li
-
Ma, Jin
-
Wei, Yong-jie
-
Wang, Chun-hui
-
Yang, Li-xin
-
Zhang, Jin-liang
-
Shi, Xiao-ming
-
Wang, Xian-liang
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Chemical Science ...
- Articles in the publication
-
Journal of Hazar ...
- By the university
-
Umeå University