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Extensive duplicati...
Extensive duplication of the Wolbachia DNA in chromosome four of Drosophila ananassae
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Klasson, Lisa (författare)
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Kumar, Nikhil (författare)
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Bromley, Robin (författare)
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visa fler...
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Sieber, Karsten (författare)
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Flowers, Melissa (författare)
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Ott, Sandra H. (författare)
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Tallon, Luke J. (författare)
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Andersson, Siv G. E. (författare)
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Hotopp, Julie C. Dunning (författare)
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visa färre...
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(utgivare)
- 2014
- 2014
- Engelska.
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Ingår i: BMC Genomics. - 1471-2164. ; 15, 1097
Abstract
Ämnesord
Stäng
- Background: Lateral gene transfer (LGT) from bacterial Wolbachia endosymbionts has been detected in similar to 20% of arthropod and nematode genome sequencing projects. Many of these transfers are large and contain a substantial part of the Wolbachia genome. Results: Here, we re-sequenced three D. ananassae genomes from Asia and the Pacific that contain large LGTs from Wolbachia. We find that multiple copies of the Wolbachia genome are transferred to the Drosophila nuclear genome in all three lines. In the D. ananassae line from Indonesia, the copies of Wolbachia DNA in the nuclear genome are nearly identical in size and sequence yielding an even coverage of mapped reads over the Wolbachia genome. In contrast, the D. ananassae lines from Hawaii and India show an uneven coverage of mapped reads over the Wolbachia genome suggesting that different parts of these LGTs are present in different copy numbers. In the Hawaii line, we find that this LGT is underrepresented in third instar larvae indicative of being heterochromatic. Fluorescence in situ hybridization of mitotic chromosomes confirms that the LGT in the Hawaii line is heterochromatic and represents similar to 20% of the sequence on chromosome 4 (dot chromosome, Muller element F). Conclusions: This collection of related lines contain large lateral gene transfers composed of multiple Wolbachia genomes that constitute >2% of the D. ananassae genome (similar to 5 Mbp) and partially explain the abnormally large size of chromosome 4 in D. ananassae.
Ämnesord
- Natural Sciences (hsv)
- Biological Sciences (hsv)
- Naturvetenskap (hsv)
- Biologiska vetenskaper (hsv)
Nyckelord
- Drosophila ananassae
- Wolbachia
- Lateral gene transfer
- Horizontal gene transfer
- Symbiosis
- Underreplication
- Heterochromatin
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