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Determination of ta...
Determination of tafenoquine in dried blood spots and plasma using LC and fluorescence detection
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- Römsing, Susanne (författare)
- Uppsala universitet,Högskolan Dalarna,Kemi,Institutionen för fysikalisk och analytisk kemi,Centrum för klinisk forskning Dalarna
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Lindegardh, Niklas (författare)
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- Bergqvist, Yngve (författare)
- Uppsala universitet,Högskolan Dalarna,Medicinsk vetenskap,Institutionen för fysikalisk och analytisk kemi
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(creator_code:org_t)
- 2011
- 2011
- Engelska.
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Ingår i: Bioanalysis. - 1757-6180 .- 1757-6199. ; 3:16, s. 1847-1853
- Relaterad länk:
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https://urn.kb.se/re...
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visa fler...
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https://doi.org/10.4...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: The growing problem of parasites developing resistance to the traditional antimalarial drugs makes the development of new effective and safe drugs crucial. Tafenoquine is a new promising antimalarial drug for prophylaxis and treatment.Results: A bioanalytical method for the determination of tafenoquine in 100 mu l of capillary blood applied onto sampling paper and in 100 mu l of plasma has been developed and validated. The Whatman 31 ET Chr paper was treated with 0.6 mol/l tartaric acid to improve the extraction recovery and solid-phase extraction was used for cleanup procedure of the blood samples. Plasma samples were precipitated with methanol. Tafenoquine and internal standard were separated on a Zorbax SB-CN column by reversed-phase LC and detected with fluorescence detection at 262 and 470 nm. The within- and between-day variations were below 10 and 14%, respectively, over the range 50-200 nmol/l for capillary blood on sampling paper and below 6 and 10% for plasma samples. The LLOQ of the method was 50 nmol/l.Conclusion: The developed method has adequate sensitivity and is highly suitable for clinical studies in dried blood spots and plasma.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Health and Welfare
- Hälsa och välfärd
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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