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Ammonium chloride i...
Ammonium chloride ingestion increases resting mRNA content but attenuates exercise-induced mRNA levels in human skeletal muscle
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Edge, J (författare)
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- Mundel, T (författare)
- Massey University, New Zealand
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- Pilegaard, H (författare)
- Centre of Inflammation and Metabolism (CIM), Department of Molecular Biology, University of Copenhagen, Denmark
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- Hawke, Emma (författare)
- Högskolan Dalarna,Idrotts- och hälsovetenskap
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- Leikas, M (författare)
- Department of Renal Medicine, Wellington Hospital, New Zealand
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- Lopez-Villalobos, N (författare)
- Massey University, New Zealand
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- da Silva Fermino de Oliviera, R (författare)
- Victoria University, Australia
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- Bishop, D (författare)
- Victoria University, Australia
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(creator_code:org_t)
- 2015-12-10
- 2015
- Engelska.
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 10:12
- Relaterad länk:
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https://doi.org/10.1...
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https://du.diva-port... (primary) (Raw object)
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Minimizing the decrease in intracellular pH during high-intensity exercise training promotes greater improvements in mitochondrial respiration. This raises the intriguing hypothesis that pH may affect the exercise-induced transcription of genes that regulate mitochondrial biogenesis. Eight males performed 10x2-min cycle intervals at 80% intensity on two occasions separated by ~2 weeks. Participants ingested either ammonium chloride (ACID) or calcium carbonate (PLA) the day before and on the day of the exercise trial in a randomized, counterbalanced order, using a crossover design. Biopsies were taken from the vastus lateralis muscle before and after exercise. The mRNA level of peroxisome proliferator-activated receptor co-activator 1α (PGC-1α), citrate synthase, cytochome c and FOXO1 was elevated at rest following ACID (P<0.05). During the PLA condition, the mRNA content of mitochondrial- and glucose-regulating proteins was elevated immediately following exercise (P<0.05). In the early phase (0–2 h) of post-exercise recovery during ACID, PGC-1α, citrate synthase, cytochome C, FOXO1, GLUT4, and HKII mRNA levels were not different from resting levels (P>0.05); the difference in PGC-1α mRNA content 2 h post-exercise between ACID and PLA was not significant (P = 0.08). Thus, metabolic acidosis abolished the early post-exercise increase of PGC-1α mRNA and the mRNA of downstream mitochondrial and glucose-regulating proteins. These findings indicate that metabolic acidosis may affect mitochondrial biogenesis, with divergent responses in resting and post-exercise skeletal muscle.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- Hälsa och välfärd
- Health and Welfare
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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