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Extraction and char...
Abstract
Ämnesord
Stäng
- Chitin nanofibers (ChNFs) were extracted from Mucor indicus by a purification method followed by a mechanical treatment, cultivated under obtained optimum culture medium conditions to improve fungal chitin production rate. A semi synthetic media containing 50 g/l glucose was used for cultivation of the fungus in shake flasks. The cell wall analysis showed that N-acetyl glucosamine (GlcNAc) content, which is an indication of chitin content, was maximum in presence of 2.5 g/l H3PO4, 2.5 g/l of NaOH, 1 g/l of yeast extract, 1 mg/l of plant hormones, and 1 ml/l of trace metals. The chemical characterizations demonstrated that the isolated fibers had a degree of deacetylation lower than of 10%, and were phosphate free. The FTIR results revealed successful removal of different materials during the purification step. The FE-SEM of fibrillated chitin illustrated an average diameter of 28 nm. Finally, X-ray diffraction results showed that the crystallinity index of nanofibers was 82%.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biomaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bio Materials (hsv//eng)
Nyckelord
- Chitin nanofiber
- Mucor indicus
- Optimized cultivation
- chitin nanoparticle
- glucose
- n acetylglucosamine
- phosphate
- chitin
- chitosan
- fungal polysaccharide
- nanofiber
- Article
- concentration (parameter)
- controlled study
- deacetylation
- decolorization
- field emission scanning electron microscopy
- Fourier transform infrared spectroscopy
- fungal biomass
- fungal cell wall
- high performance liquid chromatography
- Mucor
- nonhuman
- purification
- reaction optimization
- scanning electron microscopy
- X ray diffraction
- analysis
- biomass
- chemistry
- conditioned medium
- culture medium
- fractionation
- infrared spectroscopy
- isolation and purification
- procedures
- Chemical Fractionation
- Culture Media
- Culture Media
- Conditioned
- Fungal Polysaccharides
- Nanofibers
- Phosphates
- Spectroscopy
- Fourier Transform Infrared
- X-Ray Diffraction
- Resource Recovery
- Resursåtervinning
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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