Somatic hybridization between anther-derived dihaploid clones of potato (Solanum tuberosum L.) and the identification of hybrid plants by isozyme analysis

Waara, Sylvia,1958-Department of Plant Physiology, Uppsala University, Uppsala, Sweden (författare)

Green mesophyll protoplasts of the dihaploid potato line 198∶2 (Solanum tuberosum L.) were fused with herbicide-bleached mesophyll protoplasts of the dihaploid potato line 67∶9 using a polyethylene glycol protocol. Heterokaryons were identified under a fluorescence microscope using the dual fluorescence of carboxyfluorescein-stained, herbicide-bleached protoplasts and the autofluorescence of green mesophyll protoplasts. About 20% of the protoplasts survived the fusion treatment, and the fusion frequency was 3%-4%. Unfused and fused protoplasts were mass cultured for 6 weeks after which vigorously growing calli were selected and transferred to shoot regeneration medium. Somatic hybrids were identified by a combination of five isozyme markers, and the ploidy level was determined by flow cytometry. Out of 15 calli that regenerated shoots, 6 plants derived from 2 different calli were identified as hexaploid somatic hybrids, while one morphologically deviant plant from a third callus was identified as a mixoploid that had lost some enzyme markers after 4 months of culturing.

Tegelström, H.Department of Plant Physiology, Uppsala University, Uppsala, Sweden (författare)
Wallin, AnitaDepartment of Plant Physiology, Uppsala University, Uppsala, Sweden (författare)
Eriksson, TageDepartment of Plant Physiology, Uppsala University, Uppsala, Sweden (författare)
Department of Plant Physiology, Uppsala University, Uppsala, Sweden (creator_code:org_t)

Ingår i:Theoretical and Applied GeneticsHeidelberg : Springer Berlin/Heidelberg77:1, s. 49-560040-57521432-2242

Av författaren/redakt...: Waara, Sylvia, 1 ...; Tegelström, H.; Wallin, Anita; Eriksson, Tage

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