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Structure, organiza...
Structure, organization and expression of the genes encoding mitochondrial cytochrome c1 and the Rieske iron-sulfur protein in Chlamydomonas reinhardtii
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- Atteia, A. (författare)
- Depto. de Genética Molecular, Inst. de Fisiología Celular, Univ. Nacl. Auton. de Mex., Mexico
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- van Lis, R. (författare)
- Depto. de Genética Molecular, Inst. de Fisiología Celular, Univ. Nacl. Auton. de Mex., Mexico
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- Wetterskog, Daniel (författare)
- Department of Plant Physiology, Göteborg University, Göteborg, Sweden
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- Gutiérrez-Cirlos, E.-B. (författare)
- Department of Biochemistry, Dartmouth Medical School, Hanover, United States
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- Ongay-Larios, L. (författare)
- Unidad de Biología Molecular, Inst. de Fisiología Celular, Univ. Nacl. Auton. de Mex., Mexico
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- Franzén, Lars-Gunnar (författare)
- Högskolan i Halmstad,Växtcellbiologi: Energiomvandling i växtceller
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- González-Halphen, D. (författare)
- Depto. de Genética Molecular, Inst. de Fisiología Celular, Univ. Nacl. Auton. de Mex., Mexico
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Depto de Genética Molecular, Inst. de Fisiología Celular, Univ. Nacl. Auton. de Mex., Mexico Department of Plant Physiology, Göteborg University, Göteborg, Sweden (creator_code:org_t)
- 2003-01-15
- 2003
- Engelska.
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Ingår i: Molecular Genetics and Genomics. - : Springer Verlag. - 1617-4615 .- 1617-4623. ; 268:5, s. 637-644
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The sequence and organization of the Chlamydomonas reinhardtii genes encoding cytochrome c 1 ( Cyc1) and the Rieske-type iron-sulfur protein ( Isp), two key nucleus-encoded subunits of the mitochondrial cytochrome bc 1 complex, are presented. Southern hybridization analysis indicates that both Cyc1 and Isp are present as single-copy genes in C. reinhardtii. The Cyc1 gene spans 6404 bp and contains six introns, ranging from 178 to 1134 bp in size. The Isp gene spans 1238 bp and contains four smaller introns, ranging in length from 83 to 167 bp. In both genes, the intron/exon junctions follow the GT/AG rule. Internal conserved sequences were identified in only some of the introns in the Cyc1 gene. The levels of expression of Isp and Cyc1 genes are comparable in wild-type C. reinhardtii cells and in a mutant strain carrying a deletion in the mitochondrial gene for cytochrome b (dum-1). Nevertheless, no accumulation of the nucleus-encoded cytochrome c 1 or of core proteins I and II was observed in the membranes of the respiratory mutant. These data show that, in the green alga C. reinhardtii, the subunits of the cytochrome bc1 complex fail to assemble properly in the absence of cytochrome b.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Nyckelord
- Expression of nucleus-encoded genes
- Introns
- Internal conserved sequences
- Mitochondrial targeting sequences
- Assembly of protein complexes
- Molecular medicine (genetics and pathology)
- Molekylär medicin (genetik och patologi)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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