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Searching for genes responsible for patulin degradation in a biocontrol yeast provides insight into the basis for resistance to this mycotoxin
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- Ianiri, G. (författare)
- Dipartimento di Agricoltura, Ambiente e Alimenti, Facoltà di Agraria, Università degli Studi del Molise, Campobasso, Italy;Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri—Kansas City, Kansas City, Missouri, USA
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- Idnurm, A. (författare)
- Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri—Kansas City, Kansas City, Missouri, USA
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- Wright, Sandra A. I. (författare)
- Dipartimento di Agricoltura, Ambiente e Alimenti, Facoltà di Agraria, Università degli Studi del Molise, Campobasso, Italy
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- Durán-Patrón, R. (författare)
- Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, Puerto Real, Cádiz, Spain
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- Mannina, L. (författare)
- Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Universita' di Roma, Rome, Italy;Istituto di Metodologie Chimiche, Laboratorio di Risonanza Magnetica Annalaura Segre, CNR, Monterotondo, Rome, Italy
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- Ferracane, R. (författare)
- Dipartimento di Scienza degli Alimenti, Università di Napoli Federico II, Parco Gussone, Portici, Italy
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- Ritieni, A. (författare)
- Dipartimento di Chimica Farmaceutica e Tossicologica, Università di Napoli Federico II, Naples, Italy
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- Castoria, R. (författare)
- Dipartimento di Agricoltura, Ambiente e Alimenti, Facoltà di Agraria, Università degli Studi del Molise, Campobasso, Italy
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(creator_code:org_t)
- ASM, 2013
- 2013
- Engelska.
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Ingår i: Applied and Environmental Microbiology. - : ASM. - 0099-2240 .- 1098-5336. ; 79:9, s. 3101-3115
- Relaterad länk:
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https://aem.asm.org/...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Patulin is a mycotoxin that contaminates pome fruits and derived products worldwide. Basidiomycete yeasts belonging to the subphylum Pucciniomycotina have been identified to have the ability to degrade this molecule efficiently and have been explored through different approaches to understand this degradation process. In this study, Sporobolomyces sp. strain IAM 13481 was found to be able to degrade patulin to form two different breakdown products, desoxypatulinic acid and (Z)-ascladiol. To gain insight into the genetic basis of tolerance and degradation of patulin, more than 3,000 transfer DNA (T-DNA) insertional mutants were generated in strain IAM 13481 and screened for the inability to degrade patulin using a bioassay based on the sensitivity of Escherichia coli to patulin. Thirteen mutants showing reduced growth in the presence of patulin were isolated and further characterized. Genes disrupted in patulin-sensitive mutants included homologs of Saccharomyces cerevisiae YCK2, PAC2, DAL5, and VPS8. The patulin-sensitive mutants also exhibited hypersensitivity to reactive oxygen species as well as genotoxic and cell wall-destabilizing agents, suggesting that the inactivated genes are essential for tolerating and overcoming the initial toxicity of patulin. These results support a model whereby patulin degradation occurs through a multistep process that includes an initial tolerance to patulin that utilizes processes common to other external stresses, followed by two separate pathways for degradation.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
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