Hirudin versus heparin for use in whole blood in vitro biocompatibility models

Bexborn, Fredrik (författare): Högskolan i Kalmar,Naturvetenskapliga institutionen

Engberg, Anna E., 1982- (författare): Högskolan i Kalmar,Naturvetenskapliga institutionen,Kristina Nilsson Ekdahl

Sandholm, Kerstin (författare): Högskolan i Kalmar,Naturvetenskapliga institutionen

Mollnes, Tom Eirik (författare)

Nilsson Ekdahl, Kristina (författare): Uppsala universitet,Högskolan i Kalmar,Naturvetenskapliga institutionen,Enheten för klinisk immunologi

(creator_code:org_t)

Hoboken, NJ, US : John Wiley & Sons Inc, 2009
2009
Engelska.
Ingår i: Journal of Biomedical Materials Research. Part A. - Hoboken, NJ, US : John Wiley & Sons Inc. - 1549-3296 .- 1552-4965. ; 89A:4, s. 951-959

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Abstract Ämnesord

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Background: Heparin has traditionally been a widely used anticoagulant in blood research, but has been shown to be inappropriate for work with the complement system because of its complement-interacting properties. In this work, we have compared the effects of heparin with those of the specific thrombin inhibitor hirudin on complement and blood cells in vitro. Methods: Whole blood collected in the presence of hirudin (50 µg/mL) or heparin (1 IU/mL) was incubated in the slide chamber model. The plasma was analyzed for complement activation markers C3a and sC5b-9, and the polyvinylchloride test slides were stained for adhering cells. The integrity of the complement system was tested by incubating serum and hirudin-treated plasma in the presence of various activating agents.Results: In contrast to heparin, the addition of hirudin generally preserved the complement reactivity, and complement activation in hirudin plasma closely resembled that in normal serum. Importantly, immunochemical staining of surface-bound cells demonstrated the inducible expression of tissue factor on bound monocytes from hirudin-treated blood, an effect that was completely abolished in heparin-treated blood.Conclusion: Our results indicate that hirudin as an anticoagulant produces more physiological conditions than heparin, making hirudin well-suited for in vitro studies, especially those addressing the regulation of cellular processes.

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Av författaren/redakt...: Bexborn, Fredrik; Engberg, Anna E. ...; Sandholm, Kersti ...; Mollnes, Tom Eir ...; Hong, Jaan; Nilsson Ekdahl, ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Immunologi inom ...

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