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Effect of phenotype...
Effect of phenotype on the transcription of the genes for platelet-derived growth factor (PDGF) isoforms in human smooth muscle cells, monocyte-derived macrophages, and endothelial cells in vitro
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- Krettek, Alexandra, 1968- (författare)
- Wellenberg Lab. for Cardiovasc. Res., Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden / Wallenberg Lab. for Cardiovasc. Res., Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden
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- Fager, G. (författare)
- Wellenberg Lab. for Cardiovasc. Res., Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden
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- Lindmark, H. (författare)
- Wellenberg Lab. for Cardiovasc. Res., Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden
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visa fler...
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- Simonson, C. (författare)
- Wellenberg Lab. for Cardiovasc. Res., Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden
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- Lustig, F. (författare)
- Wellenberg Lab. for Cardiovasc. Res., Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden
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visa färre...
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Wellenberg Lab for Cardiovasc. Res., Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden / Wallenberg Lab. for Cardiovasc. Res., Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden Wellenberg Lab. for Cardiovasc. Res., Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden (creator_code:org_t)
- Lippincott Williams & Wilkins, 1997
- 1997
- Engelska.
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Ingår i: Arteriosclerosis, Thrombosis and Vascular Biology. - : Lippincott Williams & Wilkins. - 1079-5642 .- 1524-4636. ; 17:11, s. 2897-2903
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Proliferation of arterial smooth muscle cells (ASMCs) contributes considerably to enlargement of the arterial wall during atherosclerosis. The platelet-derived growth factor (PDGF) is a well-known mitogen and chemoattractant for ASMCs. Quantitative reverse transcription-polymerase chain reaction showed that cells appearing in atherosclerotic lesions, such as ASMCs, endothelial cells, and monocytes/macrophages, expressed mRNAs for both PDGF A and B chains in vitro, with the highest expression in endothelial cells. On proliferation, ASMCs and endothelial cells upregulated PDGF A mRNA. Differentiation of macrophages increased the amount of both mRNAs. Thus, the regulation of PDGF A- and B-chain expression depends on cell types and phenotypic states of the cells, which have also been found in vivo in human atherosclerotic lesions. PDGF A can be produced as short and long isoforms. The latter binds with high affinity to glycosaminoglycans. Irrespective of phenotype, only the minor part of total PDGF A mRNA consisted of the long variant in ASMCs, while endothelial cells produced 40% of total PDGF A as the long form. The differentiation of macrophages increased the production of the long PDGF A mRNA from 10% to 40%. Thus, increasing numbers of stimulated cells in the atherosclerotic lesion may increase the transcription of PDGF isoforms, and particularly of the long PDGF A isoform. Together with increasing amounts of ASMC-derived proteoglycans in developing lesions, this may contribute to accumulation of PDGF in the arterial wall matrix, resulting in prolonged stimulation of ASMCs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Nyckelord
- Differentiation
- Endothelial cells
- Macrophages
- Platelet-derived growth factor
- Smooth muscle cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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