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Comparative genome ...
Comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics
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- Rahman, Aminur, 1984- (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Systems Biology Research Center, School of Bioscience, University of Skövde, Skövde, Sweden; School of Science and Technology, Örebro University, Örebro, Sweden,Biotechnology,The Life Science Center
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- Nahar, Noor (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune, India,Systems Biology Research Center, School of Bioscience, University of Skövde, Skövde, Sweden
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- Nawani, Neelu N. (författare)
- Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, India
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- Jass, Jana, 1963- (författare)
- Örebro universitet,Institutionen för naturvetenskap och teknik,Örebro University
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- Ghosh, Sibdas (författare)
- School of Arts and Science, Iona College, New Rochelle NY, USA
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- Olsson, Björn (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Bioinformatics,Systems Biology Research Center, School of Bioscience, University of Skövde, Skövde, Sweden
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- Mandal, Abul (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Biotechnology,Systems Biology Research Center, School of Bioscience, University of Skövde, Skövde, Sweden
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(creator_code:org_t)
- Elsevier, 2015
- 2015
- Engelska.
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Ingår i: Genomics. - : Elsevier. - 0888-7543 .- 1089-8646. ; 106:6, s. 384-392
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Previously, we reported an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, isolated from an arsenic contaminated lands. Here, we have investigated its genetic composition and evolutionary history by using massively parallel sequencing and comparative analysis with other known Lysinibacillus genomes. Assembly of the sequencing reads revealed a genome of ~ 4.5 Mb in size encompassing ~ 80% of the chromosomal DNA. We found that the set of ordered contigs contains abundant regions of similarity with other Lysinibacillus genomes and clearly identifiable genome rearrangements. Furthermore, all genes of B1-CDA that were predicted be involved in its resistance to arsenic and/or other heavy metals were annotated. The presence of arsenic responsive genes was verified by PCR in vitro conditions. The findings of this study highlight the significance of this bacterium in removing arsenics and other toxic metals from the contaminated sources. The genetic mechanisms of the isolate could be used to cope with arsenic toxicity.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Miljöbioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Environmental Biotechnology (hsv//eng)
Nyckelord
- Toxic metals
- Bioremediation
- Genome sequencing
- de novo assembly
- Natural sciences
- Naturvetenskap
- Bioinformatik
- Bioinformatics
- Bioteknik
- Biotechnology
- Enviromental Science
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