Application of Plasma Desorption Mass Spectrometry to Molecular Weight Determination of Structural Protein from Insect Cuticle

Roepstorff, P.Department of Molecular Biology, Odense University, Denmark (författare)

During sequence determination of a structural protein from the cuticle of the migratory locust, Locusta migratoria, a discrepancy was found between the molecular weight calculated from the sequence data (15 323) and that estimated by SDS-gel electrophoresis (21 600). The protein contains several repeated sequences, and the discrepancy might indicate that part of the sequence was missing or that the protein contained a large prosthetic group. The molecular weight of the protein was determined by 127I-plasma desorption mass spectrometry to be 15 329±50 which confirms the sequence data and shows the molecular weight determination based on SDS-electrophoresis to be too high.

Højrup, P.Department of Molecular Biology, Odense University, Denmark (författare)
Sundqvist, B.U.R.Tandem Accelerator Laboratory, Uppsala University (författare)
Jonsson, GunnarTandem Accelerator Laboratory, Uppsala University(Swepub:kau)gunnjons (författare)
Håkansson, P.Tandem Accelerator Laboratory, Uppsala University (författare)
Andersson, S. O.Institute for Biological Chemistry A, University of Copenhagen, Denmark (författare)
Johansson, K. E.Mycoplasma Laboratory, The National Veterinary Institute, Uppsala (författare)
Department of Molecular Biology, Odense University, DenmarkTandem Accelerator Laboratory, Uppsala University (creator_code:org_t)

Av författaren/redakt...: Roepstorff, P.; Højrup, P.; Sundqvist, B.U.R ...; Jonsson, Gunnar; Håkansson, P.; Andersson, S. O.; visa fler...; Johansson, K. E.; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.