Sökning: onr:"swepub:oai:DiVA.org:kau-82499" >
Impact of stationar...
Impact of stationary-phase pore size on chromatographic performance using oligonucleotide separation as a model
-
- Bagge, Joakim (författare)
- Karlstads universitet,Institutionen för ingenjörs- och kemivetenskaper (from 2013)
-
- Enmark, Martin, 1984- (författare)
- Karlstads universitet,Institutionen för ingenjörs- och kemivetenskaper (from 2013)
-
- Lesko, Marek (författare)
- Karlstads universitet,Institutionen för ingenjörs- och kemivetenskaper (from 2013)
-
visa fler...
-
- Lime, Fredrik (författare)
- Nouryon
-
- Fornstedt, Torgny, 1957- (författare)
- Karlstads universitet,Institutionen för ingenjörs- och kemivetenskaper (from 2013)
-
- Samuelsson, Jörgen, 1971- (författare)
- Karlstads universitet,Institutionen för ingenjörs- och kemivetenskaper (from 2013)
-
visa färre...
-
(creator_code:org_t)
- Elsevier, 2020
- 2020
- Engelska.
-
Ingår i: Journal of Chromatography A. - : Elsevier. - 0021-9673 .- 1873-3778. ; 1634, s. 1-10
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://kau.diva-por... (primary) (Raw object)
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- A combined experimental and theoretical study was performed to understand how the pore size of packing materials with pores 60-300 angstrom in size affects the separation of 5-50-mer oligonucleotides. For this purpose, we developed a model in which the solutes were described as thin rods to estimate the accessible surface area of the solute as a function of the pore size and solute size. First, an analytical investigation was conducted in which we found that the selectivity increased by a factor of 2.5 when separating 5- and 15-mer oligonucleotides using packing with 300 angstrom rather than 100 angstrom pores. We complemented the analytical investigation by theoretically demonstrating how the selectivity is dependent on the column's accessible surface area as a function of solute size. In the preparative investigation, we determined adsorption isotherms for oligonucleotides using the inverse method for separations of a 9- and a 10-mer. We found that preparative columns with a 60 angstrom-pore-size packing material provided a 10% increase in productivity as compared with a 300 A packing material, although the surface area of the 60 angstrom packing is as much as five time larger.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Pore size
- Ion-pair RPLC
- Oligonucleotides
- Gradient elution
- Adsorption isotherm
- Surface area
- Kemi
- Chemistry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas