Sökning: onr:"swepub:oai:DiVA.org:kth-104700" > Proteomic Analysis ...
Fältnamn | Indikatorer | Metadata |
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000 | 03624naa a2200517 4500 | |
001 | oai:DiVA.org:kth-104700 | |
003 | SwePub | |
008 | 121109s2012 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-1047002 URI |
024 | 7 | a https://doi.org/10.1021/pr300360a2 DOI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Bock, Thomas4 aut |
245 | 1 0 | a Proteomic Analysis Reveals Drug Accessible Cell Surface N-Glycoproteins of Primary and Established Glioblastoma Cell Lines |
264 | c 2012-08-31 | |
264 | 1 | b American Chemical Society (ACS),c 2012 |
338 | a print2 rdacarrier | |
500 | a QC 20121112 | |
520 | a Glioblastoma is the most common primary Glioblastoma Cell Surface Capturing brain tumor in adults with low average survival time after diagnosis. In order to improve glioblastoma treatment, new drug-accessible targets need to be identified. Cell surface glycoproteins are prime drug targets due to their accessibility at the surface of cancer cells. To overcome the limited availability of suitable antibodies for cell surface protein detection, we performed a comprehensive mass spectrometric investigation of the glioblastoma surfaceome. Our combined cell surface capturing analysis of primary ex vivo glioblastoma cell lines in combination with established glioblastoma cell lines revealed 633 N-glycoproteins, which vastly extends the known data of surfaceome drug targets at subcellular resolution. We provide direct evidence of common glioblastoma cell surface glycoproteins and an approximate estimate of their abundances, information that could not be derived from genomic and/or transcriptomic glioblastoma studies. Apart from our pharmaceutically valuable repertoire of already and potentially drug-accessible cell surface glycoproteins, we built a mass-spectrometry-based toolbox enabling directed, sensitive, and repetitive glycoprotein measurements for clinical follow-up studies. The included Skyline Glioblastoma SRM assay library provides an elevated starting point for parallel testing of the abundance level of the detected glioblastoma surfaceome members in future drug perturbation experiments. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
653 | a glioblastoma | |
653 | a N-glycoproteins | |
653 | a cell surface accessible drug targets | |
653 | a cell surface capturing | |
653 | a N-glycoproteome SRM library | |
700 | 1 | a Moest, Hansjoerg4 aut |
700 | 1 | a Omasits, Ulrich4 aut |
700 | 1 | a Dolski, Silvia4 aut |
700 | 1 | a Lundberg, Emmau KTH,Proteomik,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u12bylj1 |
700 | 1 | a Frei, Andreas4 aut |
700 | 1 | a Hofmann, Andreas4 aut |
700 | 1 | a Bausch-Fluck, Damaris4 aut |
700 | 1 | a Jacobs, Andrea4 aut |
700 | 1 | a Krayenbuehl, Niklaus4 aut |
700 | 1 | a Uhlén, Mathiasu KTH,Proteomik,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1dulvmw |
700 | 1 | a Aebersold, Ruedi4 aut |
700 | 1 | a Frei, Karl4 aut |
700 | 1 | a Wollscheid, Bernd4 aut |
710 | 2 | a KTHb Proteomik4 org |
773 | 0 | t Journal of Proteome Researchd : American Chemical Society (ACS)g 11:10, s. 4885-4893q 11:10<4885-4893x 1535-3893x 1535-3907 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-104700 |
856 | 4 8 | u https://doi.org/10.1021/pr300360a |
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