Sökning: onr:"swepub:oai:DiVA.org:kth-129103" > Spatial organizatio...
Fältnamn | Indikatorer | Metadata |
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000 | 04300naa a2200565 4500 | |
001 | oai:DiVA.org:kth-129103 | |
003 | SwePub | |
008 | 130919s2013 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:127273516 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-1291032 URI |
024 | 7 | a https://doi.org/10.1002/cyto.a.223042 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1272735162 URI |
040 | a (SwePub)kthd (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Rönnlund, Danielu KTH,Experimentell biomolekylär fysik4 aut0 (Swepub:kth)u1vmbh2t |
245 | 1 0 | a Spatial organization of proteins in metastasizing cells |
264 | c 2013-05-08 | |
264 | 1 | b John Wiley & Sons,c 2013 |
338 | a electronic2 rdacarrier | |
500 | a QC 20130920 | |
520 | a The ability of tumor cells to invade into the surrounding tissue is linked to defective adhesive and mechanical properties of the cells, which are regulated by cell surface adhesions and the intracellular filamentous cytoskeleton, respectively. With the aim to further reveal the underlying mechanisms and provide new strategies for early cancer diagnostics, we have used ultrahigh resolution stimulated emission depletion (STED) microscopy as a means to identify metastasizing cells, based on their subcellular protein distribution patterns reflecting their specific adhesive and mechanical properties. We have compared the spatial distribution of cell-matrix adhesion sites and the vimentin filamentous systems in a matched pair of primary, normal, and metastatic human fibroblast cells. We found that the metastatic cells showed significantly increased densities and more homogenous distributions of nanoscale adhesion-related particles. Moreover, they showed an increase in the number but reduced sizes of the areas of cell-matrix adhesion complexes. The organization of the vimentin intermediate filaments was also found to be significantly different in the metastasizing cells, showing an increased entanglement and loss of directionality. Image analysis procedures were established, allowing an objective detection and characterization of these features and distinction of metastatic cells from their normal counterparts. In conclusion, our results suggest that STED microscopy provides a novel tool to identify metastasizing cells from a very sparse number of cells, based on the altered spatial distribution of the cell-matrix adhesions and intermediate filaments. | |
650 | 7 | a NATURVETENSKAPx Biologix Cellbiologi0 (SwePub)106042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Cell Biology0 (SwePub)106042 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Biofysik0 (SwePub)106032 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biophysics0 (SwePub)106032 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
653 | a STED microscopy | |
653 | a cell adhesion | |
653 | a metastasis | |
653 | a cancer | |
653 | a diagnostics | |
653 | a vimentin | |
653 | a image analysis | |
653 | a Biological Physics | |
653 | a Biologisk fysik | |
700 | 1 | a Gad, Annica K. B.u Karolinska Institutet4 aut |
700 | 1 | a Blom, Hansu KTH,Experimentell biomolekylär fysik4 aut0 (Swepub:kth)u1qhk3ta |
700 | 1 | a Aspenström, Pontusu Karolinska Institutet4 aut |
700 | 1 | a Widengren, Jerker,d 1965-u KTH,Experimentell biomolekylär fysik4 aut0 (Swepub:kth)u1i3g09c |
710 | 2 | a KTHb Experimentell biomolekylär fysik4 org |
773 | 0 | t Cytometry Part Ad : John Wiley & Sonsg 83:9, s. 855-865q 83:9<855-865x 1552-4922x 1552-4930 |
856 | 4 | u https://kth.diva-portal.org/smash/get/diva2:650299/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint |
856 | 4 | u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cyto.a.22304 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-129103 |
856 | 4 8 | u https://doi.org/10.1002/cyto.a.22304 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:127273516 |
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