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An S-selective lipa...
An S-selective lipase was created by rational redesign and the enantioselectivity increased with temperature
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- Magnusson, Anders O. (författare)
- KTH,Biokemi
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- Takwa, Mohamad (författare)
- KTH,Biokemi
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- Harnberg, Anders (författare)
- KTH,Biokemi
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- Hult, Karl (författare)
- KTH,Biokemi
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(creator_code:org_t)
- Wiley, 2005
- 2005
- Engelska.
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Ingår i: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 44:29, s. 4582-4585
- Relaterad länk:
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http://www3.intersci...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Higher activity with larger pockets: The figure shows a superposition of intermediates that occur in acyl transfer to (S)-1-phenylethanol catalyzed by Candida antarctica lipase B (CALB). Wild-type CALB cannot accomodate the phenyl group (gray) in the stereospecificity pocket and form all of the catalytically essential H bonds. The Trp 104 Ala mutation liberates the volume in yellow, the S enantiomer is easily fitted, and the specificity constant increases by a factor of 130 000.
Nyckelord
- enantioselectivity
- enzyme catalysis
- hydrolases
- protein engineering
- thermodynamics
- DYNAMIC KINETIC RESOLUTION
- SECONDARY ALCOHOLS
- STEREOSELECTIVITY
- STEREOCHEMISTRY
- RECOGNITION
- ENANTIOMERS
- CATALYSTS
- ENTROPY
- CEPACIA
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- art (ämneskategori)
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