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LIGATION-BASED MUTA...
LIGATION-BASED MUTATION DETECTION AND RCA IN SURFACE UN-MODIFIED OSTE+ POLYMER MICROFLUIDIC CHAMBERS
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- Saharil, Farizah (författare)
- KTH,Mikro- och nanosystemteknik
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Ahlford, Annika (författare)
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Kuhnemund, Malte (författare)
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Skolimowski, Maciej (författare)
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Conde, Alvaro (författare)
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Dufva, Martin (författare)
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Nilsson, Mats (författare)
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Brivio, Monica (författare)
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- Van Der Wijngaart, Wouter (författare)
- KTH,Mikro- och nanosystemteknik
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- Haraldsson, Tommy (författare)
- KTH,Mikro- och nanosystemteknik
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(creator_code:org_t)
- IEEE conference proceedings, 2013
- 2013
- Engelska.
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Ingår i: 17th IEEE International Conference on Solid-State Sensors, Actuators and Microsystems & EUROSENSORS XXVII (IEEE TRANSDUCERS 2013). - : IEEE conference proceedings. - 9781467359818 ; , s. 357-360
- Relaterad länk:
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http://ieeexplore.ie...
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https://kth.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.
Nyckelord
- Epoxy
- lab-on-a-chip
- microfluidics
- Molecular diagnostics
- off-stoichiometry thiol-ene
- OSTE
- polymer
- RCA
- rolling circle amplification
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)
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