Sökning: onr:"swepub:oai:DiVA.org:kth-14253" > A high-throughput s...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 04174naa a2200565 4500 | |
001 | oai:DiVA.org:kth-14253 | |
003 | SwePub | |
008 | 100728s2007 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:uu-97143 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-142532 URI |
024 | 7 | a https://doi.org/10.1002/pmic.2007001992 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-971432 URI |
040 | a (SwePub)kthd (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Strömberg, Sarau Uppsala universitet,Institutionen för genetik och patologi4 aut |
245 | 1 0 | a A high-throughput strategy for protein profiling in cell microarrays using automated image analysis |
264 | 1 | b Wiley,c 2007 |
338 | a print2 rdacarrier | |
500 | a QC 20100728 | |
520 | a Advances in antibody production render a growing supply of affinity reagents for immunohistochemistry (IHC), and tissue microarray (TMA) technologies facilitate simultaneous analysis of protein expression in a multitude of tissues. However, collecting validated IHC data remains a bottleneck problem, as the standard method is manual microscopical analysis. Here we present a high-throughput strategy combining IHC on a recently developed cell microarray with a novel, automated image-analysis application (TMAx). The software was evaluated on 200 digital images of IHC-stained cell spots, by comparing TMAx annotation with manual annotation performed by seven human experts. A high concordance between automated and manual annotation of staining intensity and fraction of IHC-positive cells was found. in a limited study, we also investigated the possibility to assess the correlation between mRNA and protein levels, by using TMAx output results for relative protein quantification and quantitative real-time PCR for the quantification of corresponding transcript levels. In conclusion, automated analysis of immunohistochemically stained in vitro-cultured cells in a microarray format can be used for high-throughput protein profiling, and extraction of RNA from the same cell lines provides a basis for comparing transcription and protein expression on a global scale. | |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng |
653 | a antibody proteornics | |
653 | a automated image analysis | |
653 | a cell line | |
653 | a immunohistochemistry | |
653 | a tissue microarray | |
653 | a Bioengineering | |
653 | a Bioteknik | |
653 | a MEDICINE | |
700 | 1 | a Gry Björklund, Marcusu KTH,Proteomik4 aut0 (Swepub:kth)u1dzuui2 |
700 | 1 | a Asplund, Carolineu KTH,Proteomik4 aut0 (Swepub:kth)u1g2rvfy |
700 | 1 | a Sköllermo, Annau KTH,Proteomik4 aut0 (Swepub:kth)u19pupan |
700 | 1 | a Persson, Anjau KTH,Proteomik4 aut0 (Swepub:kth)u1ub8a38 |
700 | 1 | a Wester, Kennethu Uppsala universitet,Institutionen för genetik och patologi4 aut0 (Swepub:uu)kennwest |
700 | 1 | a Kampf, Carolineu Uppsala universitet,Institutionen för genetik och patologi4 aut0 (Swepub:uu)carokamp |
700 | 1 | a Nilsson, Peteru KTH,Proteomik4 aut0 (Swepub:kth)u1ws88sk |
700 | 1 | a Andersson, Ann-Catrinu Uppsala universitet,Institutionen för genetik och patologi4 aut |
700 | 1 | a Uhlén, Mathiasu KTH,Proteomik4 aut0 (Swepub:kth)u1dulvmw |
700 | 1 | a Kononen, Juhau Beecher Instruments, Sun Prairie, WI, United States4 aut |
700 | 1 | a Pontén, Fredriku Uppsala universitet,Institutionen för genetik och patologi4 aut0 (Swepub:uu)fredpont |
700 | 1 | a Asplund, Annau Uppsala universitet,Institutionen för genetik och patologi4 aut0 (Swepub:uu)annaaspl |
710 | 2 | a Uppsala universitetb Institutionen för genetik och patologi4 org |
773 | 0 | t Proteomicsd : Wileyg 7:13, s. 2142-2150q 7:13<2142-2150x 1615-9853x 1615-9861 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-14253 |
856 | 4 8 | u https://doi.org/10.1002/pmic.200700199 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-97143 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
Kopiera och spara länken för att återkomma till aktuell vy