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Controlling acousti...
Controlling acoustic streaming in a multi-well microplate for improving live cell assays
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- Ohlin, Mathias (författare)
- KTH,Tillämpad fysik
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- Christakou, Athanasia E. (författare)
- KTH,Tillämpad fysik
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- Frisk, Thomas (författare)
- KTH,Tillämpad fysik
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visa fler...
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- Önfelt, Björn (författare)
- KTH,Tillämpad fysik
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- Wiklund, Martin (författare)
- KTH,Tillämpad fysik
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visa färre...
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(creator_code:org_t)
- 2011
- 2011
- Engelska.
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; , s. 1612-1614
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Acoustic streaming in a multi-well microplate is investigated using two different ultrasonic actuation frequency-schemes: Single-frequency and frequency-modulation. The streaming is tracked by the use of 1 μm fluorescent polymer beads and micro-particle image velocimetry. The suspension also contained human B cells for studying the acoustic trapping and aggregation performance simultaneously with the acoustic streaming. Our results show a significant difference in the acoustic streaming between the two ultrasonic actuation schemes. A rotational fluid flow speed decreased a factor of 30 when frequency-modulation was applied compared to single-frequency actuation without apparently interfering with the acoustic cell trapping function.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Acoustic streaming
- Microplate
- Mixing
- Multi-well
- Particle image velocimetry
- Ultrasound
- Cell trapping
- Fluorescent polymers
- Live cell
- Micro particle image velocimetry
- Microplates
- Multi wells
- Particle image velocimetries
- Single-frequency
- Flow visualization
- Modulation
- Ultrasonics
- Velocimeters
- Velocity measurement
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)
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