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Deterministic later...
Deterministic lateral displacement device for droplet separation by size - Towards rapid clonal selection based on droplet shrinking
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- Jönsson, Håkan (författare)
- KTH,Nanobioteknologi
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- Uhlén, Mathias (författare)
- KTH,Proteomik
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- Andersson-Svahn, Helene (författare)
- KTH,Nanobioteknologi
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(creator_code:org_t)
- 2010
- 2010
- Engelska.
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; , s. 1355-1357
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- We present a novel method for robust passive separation of microfluidic droplets by size using deterministic lateral displacement(DLD). We also show that droplets containing Saccharomyces Cervisiae shrink significantly during incubation while droplets containing only yeast media retain their size. We demonstrate the DLD device by sorting out shrunken yeast-cell containing droplets from a 40-fold excess of ∼33% larger yeast-cell-free droplets generated at the same time, suggesting that DLD might be used for clonal selection. The same device also separates 11 μm from 30μm droplets at a rate of 12000droplets/second, more than twofold faster than previously demonstrated passive hydrodynamic separation devices [1].
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- Cells
- Droplets
- High-Throughput
- Separation
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)
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