Sökning: onr:"swepub:oai:DiVA.org:kth-16046" >
Dual-color total in...
Dual-color total internal reflection fluorescence cross-correlation spectroscopy
-
Leutenegger, Marcel (författare)
-
- Blom, Hans (författare)
- KTH,Experimentell biomolekylär fysik
-
- Widengren, Jerker (författare)
- KTH,Experimentell biomolekylär fysik
-
visa fler...
-
Eggeling, Christian (författare)
-
Goesch, Michael (författare)
-
Leitgeb, Rainer A. (författare)
-
Lasser, Theo (författare)
-
visa färre...
-
(creator_code:org_t)
- SPIE-Intl Soc Optical Eng, 2006
- 2006
- Engelska.
-
Ingår i: Journal of Biomedical Optics. - : SPIE-Intl Soc Optical Eng. - 1083-3668 .- 1560-2281. ; 11:4
- Relaterad länk:
-
https://www.spiedigi...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- We present the development and first application of a novel dual-color total internal reflection (TIR) fluorescence system for single-molecule coincidence analysis and fluorescence cross-correlation spectroscopy (FCCS). As a performance analysis, we measured a synthetic DNA-binding assay, demonstrating this dual-color TIR-FCCS approach to be a suitable method for measuring coincidence assays such as biochemical binding, fusion, or signal transduction at solid/liquid interfaces. Due to the very high numerical aperture of the epi-illumination configuration, our setup provides a very high fluorescence collection efficiency resulting in a two- to three- fold increase in molecular brightness compared to conventional confocal FCCS. Further improvements have been achieved through global analysis of the spectroscopic data.
Nyckelord
- fluorescence spectroscopy
- laser-induced fluorescence
- correlation
- high count-rate
- microscopy
- molecule
- cell
- biomolecules
- kinetics
- surface
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas