Arrayed identification of DNA signatures

• Over the last few years, several initiatives have described efforts to combine previously invented techniques in molecular biology with parallel detection principles to sequence or genotype DNA signatures. The Infinium (R) system from Illumina and the Affymetrix GeneChips (R) are two systems suitable for whole-genome scoring of variable positions. However, directed candidate-gene approaches are more cost effective and several academic groups and the private sector provide techniques with moderate typing throughput combined with large sample capacity suiting these needs. Recently, whole-genome sequencing platforms based on the sequencing-by-synthesis principle were presented by 454 Life Sciences and Solexa, showing great potential as alternatives to conventional genotyping approaches. In addition to these sequencing initiatives, many efforts are pursuing novel ideas to facilitate fast and cost-effective whole genome sequencing, such as ligation-based sequencing. Reliable methods for routine resequencing of human genomes as a tool for personalized medicine, however, remain to be developed.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

array

genome-wide

genotyping

sequencing

SNP

whole genome

single nucleotide polymorphisms

allele-specific hybridization

rolling-circle amplification

refractory mutation system

polymerase chain-reaction

human-genome

padlock probes

oligonucleotide arrays

molecular beacons

mass-spectrometry

Biology

Biologi

Publikations- och innehållstyp

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